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Dendrobium candidum test-tube seedling rooting medium and preparation method thereof

A rooting medium and technology of Dendrobium candidum, applied in the field of plant tissue culture, can solve the problems of low turnover rate of tissue culture room, long culture period, increased pollution rate of test-tube seedlings, etc., and achieve stable quality of test-tube seedlings, stable pH value, pH The effect of small changes

Inactive Publication Date: 2014-10-15
上海农业科技种子有限公司 +1
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AI Technical Summary

Problems solved by technology

The long cycle of test-tube seedling culture results in low turnover rate of tissue culture room and increased contamination rate of test-tube seedlings

Method used

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Embodiment Construction

[0021] The technical solution of the present invention is illustrated below through specific examples. The raw materials and reagents used in the present invention are all commercially available. Below in conjunction with embodiment the preparation of Dendrobium officinale test-tube plantlet rooting medium is described in detail.

[0022] Taking the preparation of 40 liters of rooting medium as an example, the preparation process is as follows:

[0023] (1) Peel the bananas and potatoes, weigh 4000 grams of bananas and 2000 grams of potatoes respectively, cut the bananas and potatoes into small pieces, make a paste with a mixer, add 7-8 liters of water to heat, during the heating process, keep stirring to prevent Stick the pan until it boils, then cool to room temperature.

[0024] (2) Prepare a container with a volume of about 10 liters, add 5-6 liters of pure water into the container, and measure 2000ml of mother liquor of 20 times of macroelements, 40ml of mother liquor o...

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Abstract

The invention discloses dendrobium candidum test-tube seedling rooting medium and a preparation method thereof. The rooting medium comprises the following materials: minimal medium, 0.1-0.5 mg / L NAA, 0.4-1.0 g / L activated carbon, 100 g / L banana puree, 50 g / L mashed potatoes, 20 g / L white granulated sugar, and 5 g / L agar powder. The preparation method for the rooting medium comprises the following steps: preparing minimal medium; weighing components according to the formula of the rooting medium; preparing weighed bananas and potatoes into paste; adding water for boiling; cooling to indoor temperature; mixing the components of the prepared minimal medium, weighed NAA, activated carbon, white granulated sugar and agar powder; mixing the prepared components for constant volume; adjusting pH value to 5.9-6.0; conducting sterilization; carrying out cooling; conducting solidification; obtaining the rooting medium. The rooting medium is prepared by the components and the preparation method; cultured dendrobium candidum rooting seedlings are more stable in quality; a rooting cultivating period can be shortened to 1.5-2 months; turnover efficiency of a tissue culture room is improved by more than 30%.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a rooting medium for dendrobium officinale test-tube seedlings and a preparation method thereof. Background technique [0002] Dendrobium officinale is a perennial epiphytic herbaceous plant in the family Orchidaceae, and it is the rarest and most valuable of the medicinal plants of the genus Dendrobium. Dendrobium officinale is listed as "the first of the nine great immortal herbs in China". It has unique medicinal value. Both its stems and flowers can be used as medicine, which has the functions of nourishing the stomach and promoting body fluid, nourishing yin and clearing away heat. Modern medicine has proved that Dendrobium officinale also has the functions of anti-tumor, anti-aging, and enhancing the body's immunity. Dendrobium candidum is named for its iron-green skin. In China, Dendrobium candidum is mainly distributed in Yunnan, Zhejiang, Guangxi, Sichuan and Guizhou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈权孙建肖建梅
Owner 上海农业科技种子有限公司
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