Chicken semen diluent and preparation and utilization methods thereof

Abstract

Chicken semen diluent comprises 7.0 to 9.0 g per L of fructose, 4.0 to 6.0 g per L of sodium glutamate, 11.0 to 14.0 g per L of dipotassium phosphate, 0.5 to 0.7 g per L of monopotassium phosphate, 3.0 to 5.0 g per L of TES (N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid), 4.0 to 0.8 g per L of potassium citrate, 3.0 to 6.0 g per L of sodium acetate trihydrate, 0.2 to 0.4 g per L of anhydrous magnesium chloride, 0.05 to 0.1 g per L of lycopene. A PH (Potential of Hydrogen) value of the diluent is from 7.3 to 7.5 and the osmotic pressure is from 260 to 380 mOsm. The invention also provides preparation and utilization methods for the chicken semen diluent. The preparation and utilization methods for the chicken semen diluent are characterized in that the lycopene is added to the diluent, adjusting modes of the PH value and an osmotic pressure are changed, the diluent is stored in a cryopreservation mode, the semen deposition can be performed immediately after the chicken semen is diluted or after the cold storage, and the diluent also can be used as fundamental liquid for semen cryoprotectant. The chicken semen diluent has the advantages of being simple in preparation method, long in expiration data, free of antibiotics and stable in fertility rate.

Description

technical field [0001] The invention relates to the field of poultry breeding, and more specifically, to a diluent of chicken semen and a method for preparing and using the same. technical background [0002] At present, in the field of poultry breeding, almost all of the artificial insemination of chickens is undiluted chicken semen. On the one hand, if the undiluted chicken semen is stored for more than half an hour, it will affect the fertilization rate; on the other hand, Using undiluted chicken semen will increase the cost of poultry breeding, and if the original chicken semen can be diluted 1 to 2 times, it can greatly save the cost of breeding roosters. [0003] In addition, artificial insemination of breeder chickens in production activities often results in insufficient semen volume when the roosters are first put on the shelf, and the high death rate in the later period also causes insufficient semen volume. [0004] The artificial insemination technology of dilut...

AI Technical Summary

Problems solved by technology

[0003] In addition, artificial insemination of breeding chickens during production activities often results in insufficient semen volume when the roosters are first put on the shelf, and the high death rate in the later period also causes insufficient semen volume

[0004] The artificial insemination technology of diluted chicken semen is a complex system engineering, which is affected by the variety of chicken semen, age, feeding environment, feed, components of diluent, dilution ratio of chicken semen, insemination interval, diluent preparation method and preservation, Influenced by factors such as the amount of insemination, the depth of insemination, and the number of semen collections (Cao Jinyuan et al. 2013). In addition, Van Wambeke reported in 1967 that the diluted chicken semen had no significant effect on the fertilization rate after being stored at 5-10°C for 24 hours. However, there are still many problems in the existing technology such as the short storage period of chicken semen diluents, and even the need to prepare and use them immediately, lack of technical standards, unstable fertilization rate, etc.

[0005] Invention patent A chicken semen dilution and preservation powder and its preparation method and application (CN 101543209 A) Although a product and method for diluting and preserving chicken semen are proposed, the actual operability of the product and method is small ; and the invention patent of a diluent of chicken semen and its preparation method and application (CN 102812950 A) proposes that the diluent is stored at 4°C, has a short shelf life and antibiotics are added to the diluent, which is difficult to popularize and cause Antibiotic Residue

But at present, there is no research report on the effect of lycopene on the freezing of chicken semen

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