Technology for producing pure chlorogenic acid

A technology of pure chlorogenic acid, which is applied in the technical field of producing pure chlorogenic acid, can solve the problems of difficult membrane cleaning, high requirements for extract pretreatment, loss, etc., so as to save experimental costs and reduce subsequent processing Cost and usage reduction effect

Inactive Publication Date: 2014-10-29
SHAANXI UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] ①Water extraction and alcohol precipitation method, using the characteristics of chlorogenic acid soluble in hot water, heating and extracting with water as a solvent, and then using high-concentration alcohol to precipitate impurities such as proteins and polysaccharides. This method is simple and economical, but the process of alcohol precipitation will cause adsorption and entrainment part of chlorogenic acid, thus causing loss
The pollution of this method is relatively small, and the yield of chlorogenic acid is high, but this method has high requirements for the pretreatment of the extract, and the membrane cleaning is difficult

Method used

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  • Technology for producing pure chlorogenic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Using eucommia leaf powder and water as raw materials to prepare a concentrated solution of chlorogenic acid with a concentration of 0.8mg / mL for subsequent use; using water as an extraction solvent, dry eucommia leaf powder and water at a ratio of 1g:10mL , extracted in a water bath at 60°C for 60 minutes, the number of extractions was three times, the extracts were combined and filtered, and then the obtained filtrate was concentrated under reduced pressure and then filtered to obtain a concentrated solution of chlorogenic acid

[0034] (2) The chlorogenic acid water extraction concentrate obtained in step (1) is first purified by macroporous adsorption resin with NKAⅡ resin, washed with water to remove water-soluble impurities, and then desorbed to obtain a desorption solution, which is ethanol with a concentration of 40%. Aqueous solution, and then the desorption liquid is decompressed and concentrated to a paste to obtain a crude extract of chlorogenic acid; the v...

Embodiment 2

[0039] (1) Taking eucommia leaf powder and water as raw materials to prepare a concentrated solution of chlorogenic acid with a concentration of 1.2mg / mL for subsequent use; using water as an extraction solvent, dry eucommia leaf powder and water at a ratio of 1g:14mL , extracted in a water bath at 80°C for 40 minutes, the number of times of extraction was two times, the extracts were combined and filtered, and then the obtained filtrate was concentrated under reduced pressure and then filtered to obtain a concentrated solution of chlorogenic acid

[0040] (2) The chlorogenic acid water extraction concentrate obtained in step (1) is first purified by macroporous adsorption resin with NKAⅡ resin, washed with water to remove water-soluble impurities, and then desorbed to obtain a desorption solution. The analysis solution is ethanol with a concentration of 60%. Aqueous solution, and then concentrate the desorption liquid to a paste under reduced pressure to obtain the crude extra...

Embodiment 3

[0045] (1) Using eucommia leaf powder and water as raw materials to prepare a concentrated solution of chlorogenic acid with a concentration of 1.0mg / mL for subsequent use; using water as an extraction solvent, dry eucommia leaf powder and water at a ratio of 1g:12mL , extracted in a water bath at 70°C for 50 minutes, the number of extractions was three times, the extracts were combined and filtered, and then the obtained filtrate was concentrated under reduced pressure and then filtered to obtain a concentrated solution of chlorogenic acid

[0046] (2) The chlorogenic acid water extraction concentrate obtained in step (1) is first purified by using NKAⅡ resin for macroporous adsorption resin, then washed with water to remove water-soluble impurities, and then desorbed to obtain a desorption solution, which is ethanol with a concentration of 50%. aqueous solution, and then concentrate the desorption solution under reduced pressure to a paste to obtain the crude extract of chlor...

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Abstract

The invention relates to a technology for producing pure chlorogenic acid. The technology comprises the following steps: preparing water-extraction concentrate of chlorogenic acid from eucommia leaf powder and water, purifying the water-extraction concentrate, carrying out desorption and condensation so as to obtain a crude chlorogenic acid extract; dissolving the extract in water to obtain a chlorogenic acid water solution, adjusting the pH value of the solution, carrying out extraction so as to obtain a crude chlorogenic acid abstract, loading the abstract on a column, eluting the abstract with an organic solvent, emerging the elution solutions, and finally carrying out precipitation and recrystallization so as to obtain the pure chlorogenic acid. The extraction solvent is water, and thus the technology is green, environment-friendly, and pollution-free, and is benefit for industrial production. Before the extraction of crude chlorogenic acid, the crude chlorogenic acid is purified by resin, so the using amount of acid is prominently reduced, thus the discharge amount of waste acid is reduced, and the post-treatment cost is reduced. The technology is accord with the requirements of energy saving, discharge reduction, and green production, and is suitable for industrial production. The technology is simple, the operation is convenient, the macroporous adsorption resin and organic solvent can be repeatedly used, the comprehensive cost is low, and at the same time high purity chlorogenic acid can be obtained, so the technology is suitable for being applied to industrial production.

Description

technical field [0001] The invention relates to a process for preparing chlorogenic acid, in particular to a process for preparing pure chlorogenic acid. Background technique [0002] Eucommia ulmoides Oliv. belongs to Eucommia ulmoides. It is a traditional and precious woody medicinal plant unique to my country. The bark is traditionally used as medicine, but the bark grows slowly and peeling can easily lead to tree death, which cannot meet the market demand. However, Eucommia leaves are easy to harvest and rich in resources. The chemical composition and efficacy are similar to those of the skin, so the skin can be used as medicine, thus solving the source of Eucommia ulmoides. The problem of lack, and the content of chlorogenic acid in Eucommia leaves is much higher than that in Eucommia bark. Chlorogenic acid is an active ingredient of many Chinese patent medicines. It is contained in more than 170 Chinese patent medicines that are antibacterial, anti-inflammatory, detox...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48C07C67/56
CPCC07C67/48C07C67/56C07C69/732
Inventor 马养民冯攀张影珍任德成贾斌
Owner SHAANXI UNIV OF SCI & TECH
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