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Application of basic fuchsin and derivatives thereof in DNA detection on polyacrylamide gel

A derivative, fuchsin technology, applied in the field of DNA detection, to achieve the effect of improving safety, low cost and good reproducibility

Inactive Publication Date: 2014-10-29
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there are no reports of its use in DNA testing [2,3]

Method used

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  • Application of basic fuchsin and derivatives thereof in DNA detection on polyacrylamide gel
  • Application of basic fuchsin and derivatives thereof in DNA detection on polyacrylamide gel
  • Application of basic fuchsin and derivatives thereof in DNA detection on polyacrylamide gel

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Basic fuchsin DNA detection

[0026] figure 1 It is the chemical structural formula of basic fuchsin. The basic fuchsin DNA staining experiment is carried out by the following steps:

[0027] 1) Place the DNA sample gel after polyacrylamide electrophoresis in a staining solution for 5 minutes, wherein the staining solution is an aqueous solution containing 0.04% basic fuchsin by weight and volume, and discard the staining solution;

[0028] 2) add eluent to elute for 10s, twice, wherein the staining solution is deionized water, and discard the eluent;

[0029] 3) Add a developing solution for 2 minutes for development, wherein the developing solution is an aqueous solution containing 0.1% potassium ferricyanide (35-50° C.) by weight and volume ratio, and discard the developing solution.

[0030] 4) Stop by adding a stop solution for 2 minutes, wherein the stop solution is deionized water at 35-50°C;

[0031] According to the above method, the sodium salt, ...

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Abstract

The invention relates to a DNA detection technology and particularly relates to an application of basic fuchsin and derivatives thereof in DNA detection. The invention further provides a method of applying basic fuchsin in DNA detection on polyacrylamide gel. The method comprises the following steps: dyeing gel subjected to electrophoresis of a DNA sample in a dyeing liquid; and developing. The application provided by the invention has the advantages of high sensitivity, simple and quick operation, good reproducibility, wide linear range, safety in use, low cost and the like.

Description

technical field [0001] The invention relates to DNA detection technology, in particular to a novel dye for DNA detection. Background technique [0002] In the field of life science research, as an important macromolecule in organisms, DNA is the material basis for studying the phenomenon and essence of life. Especially with the rapid development of genomics, functional genomics and structural genomics, its application scope is increasing. Wide, mainly including: the identification of specific gene functions, new drug screening, disease diagnosis, drug action mechanism, biological development and changes in various periods, etc., it has penetrated into almost all fields of life sciences. Therefore, the study of DNA is of great significance. At present, polyacrylamide gel electrophoresis is a common method for efficiently separating and analyzing DNA molecules, making the detection technology of DNA on the gel play a vital role in the related research of DNA [1]. [0003] Th...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCG01N1/30G01N2001/302
Inventor 金利泰周旋陈茂丛维涛朱忠欣
Owner WENZHOU UNIVERSITY
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