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Stable troponin I detection kit

A technology for detecting reagents and troponin, which is applied in the field of troponin I detection kits to achieve high antibody activity and good accuracy

Active Publication Date: 2014-11-12
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The immunological activity of the antibody bound to the surface of latex particles determines the performance index of the kit prepared with it as the raw material. The conventional troponin I detection kit uses sodium chloride to ensure the ion balance, but the immunological activity of the antibody is still With the gradual attenuation of storage time, this greatly restricts the application of latex-enhanced immunoturbidimetry to the detection of troponin I

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Troponin I detection reagent components and concentrations are

[0023] Component 1:

[0024] Glycine buffer solution with pH 7.6 25mmol / L

[0025] Sodium chloride 150mmol / L

[0026] Sucrose Esters 1%;

[0027] Prepared by dissolving in distilled water;

[0028] Component 2:

[0029] Glycine buffer solution with pH 7.6 25mmol / L

[0030] Goat anti-human TnI antibody coated latex particles 3%.

[0031] Prepared by dissolving in distilled water.

[0032] The application process of the troponin I detection reagent is as follows: use an automatic biochemical analyzer with dual reagent functions, such as Toshiba 40 automatic analyzer, and the operation is shown in Table 1:

[0033] Table 1 Detection method of troponin I detection reagent

[0034]

[0035] Calculation: troponin I concentration = (?A determination ÷?A standard) × C standard

Embodiment 2

[0037] Troponin I detection reagent components and concentrations are

[0038] component 1

[0039] Glycine buffer solution with pH 7.6 25mmol / L

[0040] Sodium chloride 150mmol / L

[0041] Prepared by dissolving in distilled water;

[0042] Component 2:

[0043] Glycine buffer solution with pH 7.6 25mmol / L

[0044] Sucrose 3%

[0045] Glycerin 8%

[0046] EDTA0.1%

[0047] Goat anti-human TnI antibody coated latex particles 3%

[0048] Prepared by dissolving in distilled water.

[0049] Using method is the same as embodiment 1.

Embodiment 3

[0051] Troponin I detection reagent components and concentrations are:

[0052] Component 1:

[0053] Glycine buffer solution with pH 7.6 25mmol / L

[0054] Sodium chloride 150mmol / L

[0055] Sucrose Esters 1%;

[0056] Prepared by dissolving in distilled water;

[0057] Component 2:

[0058] Glycine buffer solution with pH 7.6 25mmol / L

[0059] Sucrose 3%

[0060] Glycerin 8%

[0061] EDTA0.1%

[0062] Goat anti-human TnI antibody coated latex particles 3%

[0063] Prepared by dissolving in distilled water.

[0064] Using method is the same as embodiment 1.

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Abstract

The invention relates to a stable troponin I detection kit. The content of raw materials of each component of the stable troponin I detection kit is as follows: a component 1 comprises 25mmol / L glycine buffer solution with the pH value of 7.6, 150mmol / L sodium chloride and 1 percent of sucrose ester; a component 2 comprises 25mmol / L glycine buffer solution with the pH value of 7.6, 3 percent of sucrose, 8 percent of glycerinum, 0.1 percent of EDTA (ethylene diamine tetraacetic acid) and 3 percent of goat anti human TnI antibody cladded latex particles. The stable troponin I detection kit is good in accuracy and can be stably stored for one year at the temperature of 2 to 8 DEG C.

Description

[0001] technical field [0002] The invention relates to a stable troponin I detection kit, which belongs to the technical field of clinical in vitro detection reagents. [0003] Background technique [0004] Cardiac troponin was first reported in 1987 when Cummins et al. diagnosed acute myocardial infarction (acute myocardial infarction, AMI). Troponin (Tn) is the main structural contractile protein of the myocardium and consists of three subunits: troponin c (TnC), troponin T (TnT) and troponin I (TnI). Among them, TnI is divided into three subtypes: fast skeletal muscle subtype (frnI), slow skeletal muscle subtype (sTnI) and cardiac muscle subtype (cTnI), but only cTnI exists in the myocardium after 9 months of birth. cTnI does not have any subtypes, its molecular weight is 24kDa, and it consists of 209 amino acids. More than 40% of its amino acid sequences are heterologous to other subtypes, and a sequence of 32 amino acids is extended from the amino terminal; so cT...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/324
Inventor 甘宜梧张永英谭柏清李静王绮
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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