Method for reducing organic acid content of fruit wine
An organic acid and fruit wine technology, which is applied in the direction of microorganism-based methods, wine preparation, alcoholic beverage preparation, etc., can solve the problem of undiscovered fungal hyphae, etc., and achieve the effect of reducing organic acids
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Embodiment 1
[0026] Mushroom hyphae or spores were inoculated into liquid medium (starch 10g / L, sucrose 20g / L, peptone 0.5g / L, K 2 HPO 4 0.5g / L,V B1 0.001g / L, pH 5-6), temperature 28°C, aerated culture for 3-4 days. The hyphae balls were obtained by filtration, washed twice with sterile water, and stored at 4°C until use. The pre-fermentation of navel orange fruit wine is over, and the reducing sugar is lower than 4g / L. When pouring wine, add 30% (W / V) shiitake mushroom mycelium to the wine storage tank, keep the wine temperature at 15-25°C, and stir once a day. After 2-3 days, the citric acid content of the wine sample decreased from 12.0% to 7.5%. Lower the temperature of the wine to 10-15°C, and pour the wine again after standing for 3 days. After 30 days of post-fermentation, the acidity and alcohol content of the wine sample were 6.32% (W / V) and 13.55% (V / V) respectively. The flavor was normal, and there was no bad smell caused by the mushroom hyphae.
Embodiment 2
[0028] Aspergillus oryzae spores were inoculated with Cha's liquid medium (sodium nitrate 3g / L, dipotassium hydrogen phosphate 1g / L, magnesium sulfate (MgSO4 7H2O) 0.5g / L, potassium chloride 0.5g / L, ferrous sulfate 0.01g / L L, sucrose 30g / L), temperature 28°C, aerated culture for 2-3 days, filter to obtain mycelial balls, wash twice with sterile water, and store at 4°C until use. The pre-fermentation of navel orange fruit wine is over, and the reducing sugar is lower than 4g / L. When pouring wine, add 30% (W / V) Aspergillus oryzae mycelia to the wine storage tank, keep the wine temperature at 15-25°C, and stir once a day. After 2-3 days, the citric acid content of the wine sample decreased from 12.0% to 7.0%. Lower the temperature of the wine to 10-15°C, and pour the wine again after standing for 3 days. After 30 days of post-fermentation, the acidity and alcohol content of the wine sample were 7.30% (W / V) and 13.0% (V / V) respectively, and the flavor was normal.
Embodiment 3
[0030]Monascus spores were inoculated into liquid medium: 70.0 g / L soluble starch, 25.0 g / L peptone, 1.0 g / L MgSO4 7H2O, cultured aerobically for 72 hours until mycelium flourished, collected mycelium balls by filtration, and used Bacterial water washing 2 times. After the pre-fermentation period of plum fruit wine, the reducing sugar was lower than 4g / L. When pouring wine, add 20% (W / V) Monascus mycelium into the wine storage tank, store the wine at 25°C, stir once a day, and start to stand for several days after three days, and pour the wine again after precipitation. Post-fermentation for 30 days. The acidity (calculated by malic acid) of the wine sample decreased from 14.2% to 8.3%, the alcohol content was basically unchanged, and the flavor was normal.
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