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Application of earthworm extract to medicines

A technology of extracts and earthworms, applied in the fields of biomedicine and preventive medicine, can solve the problems of lung fibrosis of earthworm extracts that have not been disclosed, and achieve excellent preventive effects

Active Publication Date: 2014-12-03
倪春辉 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing literature does not disclose whether the earthworm extract can treat pulmonary fibrosis, that is to say, the existing research does not disclose whether the earthworm extract can treat respiratory system dysfunction and lung tissue fibrosis caused by generated dust, especially free silica. developmental interventions

Method used

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  • Application of earthworm extract to medicines
  • Application of earthworm extract to medicines
  • Application of earthworm extract to medicines

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0026] Experimental example 1 Evaluation of the effect of earthworm extract on the development of silicosis in mice

[0027] 1. Purpose of the experiment

[0028] To observe the effect of earthworm extract on silica dust-induced pulmonary fibrosis, and to provide a theoretical basis for clinical application.

[0029] 2. Experimental materials

[0030] Animals: SPF grade healthy adult male C57BL / 6 mice, 4-6 weeks old, weighing 20-25 grams, purchased from the Animal Center of Nanjing Medical University, free to drink and eat during the administration;

[0031] Reagents: crystalline silica standard was purchased from Sigma; 75vt.% alcohol solution; 1wt.% sodium pentobarbital; 0.4wt.% iodophor; normal saline; 75vt.% ethanol;

[0032] Drugs: The earthworm extract is the extract of Cantonia glabrata (the activity of various enzymes is represented by the activity of lumbrokinase, about 220000U / mg).

[0033] 3. Experimental method

[0034] (1) Establishment and treatment of mouse ...

experiment example 2

[0057] Experimental example 2 Evaluation of the effect of giving earthworm extracts to the digestive tract to prevent the occurrence and development of silicosis in mice

[0058] 1. Purpose of the experiment

[0059] To observe the effect of earthworm extract on preventing silica dust-induced pulmonary fibrosis, and to provide a theoretical basis for clinical application.

[0060] 2. Experimental materials

[0061] Animal: Same as Experiment 1

[0062] Reagent: same as Experiment 1

[0063] Drugs: The earthworm extract is guangdilong extract (lumbrokinase about 220000U / g); pirfenidone

[0064] Pirfenidone is a new targeted anti-fibrotic drug, and pirfenidone has been used as a clinically recommended drug for the treatment of idiopathic pulmonary fibrosis (IPF) in European countries and Japan. Although its exact mechanism is not very clear, it is relatively clear that it mainly inhibits the TGF-β pathway through targeting. Studies have found that pirfenidone can reduce the...

experiment example 3

[0077] Experimental example 3 Evaluation of the effect of earthworm extract on the development of silicosis in mice

[0078] 1. Purpose of the experiment

[0079] To observe the effect of earthworm extract on silica dust-induced pulmonary fibrosis, and to provide a theoretical basis for clinical application.

[0080] 2. Experimental materials

[0081] ditto

[0082] 3. Experimental method

[0083] (1) Establishment and treatment of mouse silicosis model: C57BL / 6 mice were weighed, numbered, and randomly grouped. See Table 1 for specific groups. Anesthetized with 0.2 mL of 1% pentobarbital by intraperitoneal injection, fixed on the test bench in supine position, and 75% ethanol was used to disinfect the neck after hair removal. The skin was incised, bluntly dissected, and the trachea was exposed layer by layer. A 1mL syringe was inserted into the trachea toward the heart through the gap between the two tracheal cartilage rings. The blank control group was injected with 50 ...

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Abstract

The invention discloses application of earthworm extract to medicines. The earthworm extract is prepared by the following steps: cleaning fresh pheretimaasperfillm, discharging mud by clear water for 30 minutes, adding a 0.02mol / L phosphate buffer with pH value of 7.3 and a 3wt% sodium benzoate aqueous solution which have the same volume as the clear water, keeping the constant temperature of 37 DEG C, centrifuging at the speed of 8,000r / min and the temperature of 4 DEG C for 30 minutes, adding ethanol into a supernatant, staying overnight at 4 DEG C, centrifuging, drying settlings in vacuum, and storing; purifying a crude extract by adopting an ion-exchange column chromatography method, freeze-drying, and storing. Compared with PFD, the earthworm extract has the characteristics that the treatment and prevention effects of the earthworm extract are equivalent to those of the PFD, and the lumbrukinase activity in the unit weight of earthworm extract reaches up to 220,000U / mg by adopting the extraction method, so that the effective dosage of the earthworm extract is remarkably lower than that of the PFD.

Description

technical field [0001] The invention belongs to the fields of biomedicine and preventive medicine, and in particular relates to the medical application of earthworm extracts. Background technique [0002] Pneumoconiosis is a systemic disease mainly caused by irreversible fibrosis of lung tissue caused by long-term inhalation of productive dust. Among occupational diseases in my country, pneumoconiosis ranks first in both morbidity and mortality. According to official statistics from the National Health and Family Planning Commission, there were 26,393 cases of occupational diseases reported nationwide in 2013, and pneumoconiosis accounted for 87.72% of the total cases, while silicosis and coal workers' pneumoconiosis accounted for 95.24%. Coal workers' pneumoconiosis includes coal lung, silicosis and coal silicosis. The vast majority of coal workers' pneumoconiosis in my country belongs to coal silicosis, accounting for about 90%. Epidemiological surveys show that occupati...

Claims

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Application Information

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IPC IPC(8): A61K35/56A61P11/00
Inventor 倪春辉王婷
Owner 倪春辉
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