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Prostatic cancer-resistant mussel glycoprotein and preparation and application thereof

A glycoprotein, mussel technology, applied in the field of chemical engineering, can solve problems that have not yet been reported

Inactive Publication Date: 2014-12-03
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] During the search process, the applicant found that mussels were used as raw materials, and the research on the preparation of glycoproteins by water extraction, ion exchange resin column chromatography, gel column chromatography and protein rapid purification system was in a blank stage. For its anti-prostate cancer field The application has not yet been reported

Method used

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  • Prostatic cancer-resistant mussel glycoprotein and preparation and application thereof
  • Prostatic cancer-resistant mussel glycoprotein and preparation and application thereof
  • Prostatic cancer-resistant mussel glycoprotein and preparation and application thereof

Examples

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Embodiment 1

[0023] Instruments and equipment used in the present invention:

[0024] AKTA purifier 100 (Amersia, Sweden);

[0025] Agilent 1200 high performance liquid chromatography (Agilent, USA);

[0026] Milli-Q system (Millipore, USA);

[0027] DEAE Sepharose F. F and Sepharose 4B column chromatography equipment (Pharmacia, Sweden);

[0028] FDU-1200 small freeze dryer (Japan EYELA company);

[0029] DS-21 high-speed tissue grinder (Shanghai Specimen Model Factory);

[0030] UV-22000 ultraviolet spectrophotometer (Unico Instrument (Shanghai) Co., Ltd.);

[0031] DYY-22C electrophoresis instrument (Beijing Liuyi Instrument Factory);

[0032] Hei-VAP rotary evaporator (Heidolph, Germany);

[0033] BSZ-100 Automatic Part Collector (Shanghai Qite Analytical Instrument Co., Ltd.)

[0034] LXB centrifuge (Shanghai Medical Analytical Instrument Factory);

[0035] SuperdexTM 75 preparative column (Amersia, Sweden)

[0036] Reagents and raw materials used in the present invention:

...

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Abstract

The invention discloses a mussel glycoprotein and a preparation method and an application thereof. The mussel glycoprotein disclosed by the invention is prepared by the following steps: carrying out tissue homogenization of mussel soft tissues; extracting by a buffer solution; carrying out ion exchange column chromatography; carrying out gel column chromatography; purifying by virtue of a fast protein purifying system; concentrating; and freeze-drying. The molecular weight of the mussel glycoprotein is about 16.9kDa and the content of sugar and proteins is respectively 23.54% and 76.46%. The mussel glycoprotein contains O-glucosidic bonds and N-glucosidic bonds. Monosaccharide consists of D-glucose, L-arabinose, D-galactose, D-fructose and D-mannose in a corresponding proportion of 1.00 to 2.01 to 6.93 to 7.27 to 4.05. The protein consists of 15 amino acids. The mussel glycoprotein is remarkable in activity, can be clinically used as an agiogenesis inhibitor and can be used for treating tumor diseases such as prostatic cancer.

Description

technical field [0001] The invention belongs to the technical field of chemical engineering, and in particular relates to an anti-prostate cancer mussel glycoprotein and its preparation and application. Background technique [0002] Mussels are bivalve molluscs, and they are also important cultured shellfish in my country. In northern my country, mussels are commonly called Haihong, in the south they are commonly called mussels, and their dried products are commonly called mussels. They are one of the famous seafood with both medicinal and edible uses. There are several kinds of mussels produced in China, such as purple mussels, thick-shell mussels, and emerald mussels. In recent years, some researchers have studied mussel active substances, such as using mussels to prepare functional peptides and active polysaccharides, using mussel fat-soluble components to prepare anti-inflammatory drugs, etc., providing a theoretical basis for the high-value utilization of mussels. Tec...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/18C07K1/16A61K38/17A61P35/00
CPCC07K14/43504A61K38/00
Inventor 迟长凤王斌陈荫李涛
Owner ZHEJIANG OCEAN UNIV
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