Measurement method of nitrogen and oxygen stable isotope of nitrate in seawater
A stable isotope and nitrate nitrogen technology, which is applied in the field of nitrate pollution control and control in water bodies, can solve the problems of relatively harsh cultivation conditions for denitrifying bacteria, affect the results of sample pretreatment, and expensive instruments, etc., and achieve the effect of bacterial enrichment Good, better culture effect, stable bacterial growth effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0022] 1. Enrichment culture of denitrifying bacteria
[0023] The formula of solid beef extract peptone medium is: 3.0g beef extract, 10.0g peptone, 5.0g sodium chloride, 15.0g agar, 1.0L deionized water. The formula of liquid beef extract peptone culture medium is: 3.0g beef extract, 10.0g peptone, 5.0g sodium chloride, 1.0L deionized water.
[0024] Prepare solid beef extract peptone medium, and prepare a solid plate by high temperature and high pressure sterilization. Streak inoculation without N 2 For the denitrifying bacteria with O reductase activity, after three days of cultivation, take a single colony and inoculate it into 5 mL liquid beef extract peptone culture solution that has been sterilized, continue to cultivate it on a shaker at 30 °C and 120 rpm for one day, and then transfer to 600 mL denitrified bacteria. Bacteria were enriched in the liquid beef extract peptone culture solution at 30°C and 120 rpm.
[0025] The no N 2 The denitrifying bacteria with O ...
Embodiment 2
[0039] 1. Enrichment culture of denitrifying bacteria
[0040] The formula of solid beef extract peptone medium is: 3.0g beef extract, 10.0g peptone, 5.0g sodium chloride, 15.0g agar, 1.0L deionized water. The formula of liquid beef extract peptone culture medium is: 3.0g beef extract, 10.0g peptone, 5.0g sodium chloride, 1.0L deionized water.
[0041] Prepare solid beef extract peptone medium, and prepare a solid plate by high temperature and high pressure sterilization. Streak inoculation without N 2 For the denitrifying bacteria with O reductase activity, after three days of cultivation, take a single colony and inoculate it into 5 mL liquid beef extract peptone culture solution that has been sterilized, continue to cultivate it on a shaker at 30 °C and 120 rpm for one day, and then transfer to 600 mL denitrified bacteria. Bacteria were enriched in the liquid beef extract peptone culture solution at 30°C and 120 rpm.
[0042] The no N 2 The denitrifying bacteria with O ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com