Microfluidic chip and cell screening method for screening specific cells

Abstract

The invention discloses a microfluidic chip and a cell screening method for screening specific cells. The chip comprises a substrate, a cavity and an acoustic excitation source, wherein the cavity comprises a middle channel, an inlet and an outlet; the acoustic excitation source is positioned on the substrate and at two sides of the middle channel and comprises two parts which extend to a direction of the outlet along the middle channel, wherein the corresponding acoustic resonance frequency of the second part close to the outlet is integer multiples of the corresponding acoustic resonance frequency of the first part far away from the outlet. Based on an acoustic streaming effect formed by targeted microbubbles and asymmetric sound fields, the microbubbles are adhered to the surfaces of the specific cells, so that the enriched cells which flow past the first part can still move in a straight line and cannot diffuse due to a laminar flow characteristic of microfluidics, and the specific cells bonded with the targeted microbubbles can shift because the acoustic resonance frequency of the second part is different from that of the first part. Thus, the screening of the specificity of the specific cells in blood is realized.

Description

technical field [0001] The invention relates to the technical field of cell analysis, in particular to a microfluidic chip for screening specific cells and a cell screening method. Background technique [0002] Molecular biology and clinical studies have shown that some cancers diagnosed as early stages actually have distant metastases, that is, tumor micrometastases, which are difficult to detect by conventional imaging, histology or cytology methods. Tumor micrometastasis can form micrometastasis lesions in the tissues and organs of the whole body through the hematogenous and lymphatic pathways, and the development of lymph node metastasis will eventually enter the blood circulation to form circulating tumor cells (Circulating Tumor Cells, CTCs), leading to systemic metastasis of the primary tumor. tendency. The detection of CTCs is helpful for early detection of tumor micrometastasis, monitoring of postoperative recurrence, evaluation of curative effect and prognosis, or...

AI Technical Summary

Problems solved by technology

However, the acoustic method lacks specificity, and the particle size of CTCs is aliased with the cell particle size in blood (such as figure 1 shown), it is difficult to screen CTCs cells

[0009] In summary, although there are many methods to screen CTCs, each method has certain shortcomings and deficiencies, which directly affect the efficiency of cell screening and cell activity, making it difficult to achieve early diagnosis of cancer metastasis.

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