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Nasal cavity secretion treating liquid and treating method thereof

A treatment method and secretion technology, which is applied in the field of clinical medicine, can solve the problems of inability to carry out immunohistochemical staining and the inability to disperse target cells, and achieve the effect of uniform cell distribution, thin and uniform cell distribution

Active Publication Date: 2014-12-17
张罗 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional cytological examination of nasal secretions is to directly smear the secretions or mucous membrane scrapings on the center of the glass slide, and then stain them directly after drying and fixing. However, nasal secretions contain a lot of impurities and viscous substances, which lead to The target cells cannot be evenly dispersed on the glass slide, and immunohistochemical staining cannot be performed; in addition, the impurities in the secretion will also have a great impact on the staining effect of the smear and the correct judgment of the result

Method used

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  • Nasal cavity secretion treating liquid and treating method thereof
  • Nasal cavity secretion treating liquid and treating method thereof
  • Nasal cavity secretion treating liquid and treating method thereof

Examples

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Effect test

Embodiment 1

[0073] The cleaning solution includes ethylenediaminetetraacetic acid, methanol, disodium hydrogen phosphate, potassium dihydrogen phosphate, sodium chloride, potassium chloride, redistilled water and hydrochloric acid, and the quality of said ethylenediaminetetraacetic acid and said redistilled water Volume ratio is 0.3g / L, and the volume ratio of described methanol and described redistilled water is 1:1, and the mass volume ratio of described disodium hydrogen phosphate and described redistilled water is 1.44g / L, and described phosphoric acid The mass volume ratio of potassium dihydrogen to the redistilled water is 0.24g / L, the mass volume ratio of the sodium chloride to the redistilled water is 8g / L, and the potassium chloride to the redistilled water The mass volume ratio of the hydrochloric acid is 0.2g / L, and the pH of the cleaning solution is adjusted to 7.3 by the hydrochloric acid;

[0074] The digestive juice comprises dithiothreitol, disodium hydrogen phosphate, pot...

Embodiment 2

[0083] The cleaning solution includes ethylenediaminetetraacetic acid, methanol, disodium hydrogen phosphate, potassium dihydrogen phosphate, sodium chloride, potassium chloride, redistilled water and hydrochloric acid, and the quality of said ethylenediaminetetraacetic acid and said redistilled water The volume ratio is 0.2g / L, the volume ratio of the methanol and the redistilled water is 1:0.5, the mass volume ratio of the disodium hydrogen phosphate and the redistilled water is 1.0g / L, the phosphoric acid The mass volume ratio of potassium dihydrogen to the redistilled water is 0.2g / L, the mass volume ratio of the sodium chloride to the redistilled water is 7g / L, and the potassium chloride to the redistilled water The mass volume ratio of the hydrochloric acid is 0.1g / L, and the pH of the cleaning solution is adjusted to 7.2 by the hydrochloric acid;

[0084] The digestive juice comprises dithiothreitol, disodium hydrogen phosphate, potassium dihydrogen phosphate, sodium ch...

Embodiment 3

[0093] The cleaning solution includes ethylenediaminetetraacetic acid, methanol, disodium hydrogen phosphate, potassium dihydrogen phosphate, sodium chloride, potassium chloride, redistilled water and hydrochloric acid, and the quality of said ethylenediaminetetraacetic acid and said redistilled water Volume ratio is 0.4g / L, and the volume ratio of described methanol and described redistilled water is 1:1.5, and the mass volume ratio of described disodium hydrogen phosphate and described redistilled water is 1.5g / L, and described phosphoric acid The mass volume ratio of potassium dihydrogen to the redistilled water is 0.3g / L, the mass volume ratio of the sodium chloride to the redistilled water is 9g / L, and the potassium chloride to the redistilled water The mass volume ratio of the hydrochloric acid is 0.3g / L, and the pH of the cleaning solution is adjusted to 7.4 by the hydrochloric acid;

[0094] The digestive juice comprises dithiothreitol, disodium hydrogen phosphate, pot...

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Abstract

The invention relates to the field of clinical medicine, particular to nasal cavity secretion treating liquid and a treating method thereof. The nasal cavity secretion treating liquid comprises cleaning fluid, digestive fluid and protective solution which are used sequentially. The treating method includes the steps of suspension of nasal cavity secretion with the cleaning liquid, splitting decomposition of viscous components of the nasal cavity secretion with the digestive fluid, filtration and centrifugation with a cell strainer to remove impurities and viscous substances, addition of the protective solution, uniformly dispersion of cells in the preservative solution, transference of uniformly-dispersed cells to the cell strainer, transference of cells on the cell strainer to a glass slide, and fixing to obtain a clear and beautiful cell smear of thin and uniformly-distributed cells. The uniformly-distributed cells can be observed through a microscope, the cells are representative and are not overlapped, the influence of mucus and impurities on the observation of the cells is removed, and the immunohistochemical staining can be directly performed.

Description

technical field [0001] The invention relates to the field of clinical medicine, in particular to a treatment liquid for nasal secretion and a treatment method thereof. Background technique [0002] Cytological examination of nasal secretion is of great significance for clinical diagnosis and treatment. The traditional cytological examination of nasal secretions is to directly smear the secretions or mucous membrane scrapings on the center of the glass slide, and then stain them directly after drying and fixing. However, nasal secretions contain a lot of impurities and viscous substances, which lead to The target cells cannot be evenly dispersed on the glass slide, and immunohistochemical staining cannot be performed; in addition, the impurities in the secretion will also have a great impact on the staining effect of the smear and the correct judgment of the result. Contents of the invention [0003] The object of the present invention is to provide a treatment liquid for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38G01N1/34G01N1/28G01N1/30
Inventor 张罗佘文煜杨军
Owner 张罗