Method for extracting coriolus versicolor polysaccharides

An extraction method, the technology of versicolor versicolor polysaccharides, applied in the field of bioengineering, can solve the problems of large amount of extraction liquid and large energy consumption, and achieve the effect of cheap solvent, avoiding interference, and high polysaccharide extraction rate

Inactive Publication Date: 2014-12-24
中山安荞生物科技有限公司
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current water leaching method takes 5-10 hours to extract, and the amount of leaching solution is large, which consumes a lot of energy, and the yield of Yunzhi polysaccharide is only about 7.81%.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting Yunzhi polysaccharides, comprising the following steps:

[0027] (1) Sample pretreatment: take a proper amount of versicolor versicolor, rinse it, dry and grind it into a 40-mesh coarse powder, and set aside;

[0028] (2) Degrease 5g of versicolor versicolor powder in step (1), add 10ml of 2% sodium carbonate solution for ultrasonic extraction for 45 minutes, first filter with a 0.05um microporous membrane, the inlet pressure is 2bar, the outlet pressure is 0bar, and the filtrate is collected , and then the filtrate is filtered by an ultrafiltration membrane with a molecular weight cut-off of 800Da, the operating temperature is 38 ° C, the inlet pressure is 1 bar, and the outlet pressure is 1 bar, and the filtrate after ultrafiltration is collected;

[0029] (3) Add the filtrate after ultrafiltration in step (1) to 10ml of absolute ethanol, mix and centrifuge, discard the supernatant, add 10ml of 70% ethanol to the precipitate, mix well, centrifu...

Embodiment 2

[0035] A method for extracting Yunzhi polysaccharides, comprising the following steps:

[0036] (1) Sample pretreatment: take a proper amount of versicolor versicolor, rinse it, dry and grind it into a 40-mesh coarse powder, and set aside;

[0037] (2) Degrease 10g of versicolor versicolor powder in step (1), add 20ml of 5% sodium carbonate solution for ultrasonic extraction for 55 minutes, first filter with a 1um microporous membrane, the inlet pressure is 6bar, the outlet pressure is 5bar, and the filtrate is collected. Then the filtrate is filtered by an ultrafiltration membrane with a molecular weight cut-off of 1000Da, the operating temperature is 40°C, the inlet pressure is 8bar, and the outlet pressure is 3bar, and the filtrate after ultrafiltration is collected;

[0038] (3) Add the filtrate after ultrafiltration in step (1) to 20ml of absolute ethanol, mix and centrifuge, discard the supernatant, add 20ml of 80% ethanol to the precipitate, mix well, centrifuge, discar...

Embodiment 3

[0044] A method for extracting Yunzhi polysaccharides, comprising the following steps:

[0045] (1) Sample pretreatment: take a proper amount of versicolor versicolor, rinse it, dry and grind it into a 40-mesh coarse powder, and set aside;

[0046] (2) Degrease 8g of Versicolor versicolor powder in step (1), add 15ml of 4% sodium carbonate solution for ultrasonic extraction for 50min, first filter with a 0.05~1um microporous membrane, the inlet pressure is 4bar, the outlet pressure is 3bar, and the filtrate is collected , and then the filtrate is filtered by an ultrafiltration membrane with a molecular weight cut-off of 900Da, the operating temperature is 39°C, the inlet pressure is 5bar, and the outlet pressure is 2bar, and the filtrate after ultrafiltration is collected;

[0047] (3) Add the filtrate after ultrafiltration in step (1) to 15ml of absolute ethanol, mix and centrifuge, discard the supernatant, add 15ml of 75% ethanol to the precipitate, mix, centrifuge, discard ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for extracting coriolus versicolor polysaccharides. The method comprises the following steps: firstly, preprocessing a sample, namely rinsing, drying and grinding the sample; secondly, adding the sample into a sodium carbonate solution, performing ultrasonic extraction, and filtering through a microporous filter membrane and a superporous filter membrane successively; thirdly, centrifuging filter liquor twice, adding the sodium carbonate solution into sediments for extraction, centrifuging and concentrating to prepare concentrated pulp; finally, drying the concentrated pulp until the water content is 3%-8%. According to the method, coriolus versicolor is subjected to ultrasonic extraction, so that the highest polysaccharide extraction rate can be obtained, the operation time can be greatly shortened, and the extraction efficiency is improved; in the extraction process of the coriolus versicolor polysaccharides, the 2%-5% sodium carbonate solution is adopted as a solvent, so that the extraction effect is good; the solvent is low in price, safe, harmless, economic and environmental friendly; moreover, the sample is subjected to grinding, screening, ultrasonic extraction and two-time filtering, so that the interference of related impurities on the extraction process is avoided.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for extracting polysaccharides from versicolor versicolor. Background technique [0002] Coriolus versicolor (Coriolus versicolor), also known as Coriolus versicolor, Coriolus versicolor, etc., belongs to the phylum Fungi, Basidiomycotina, Polyporaceae, and the genus Coriolus. Yunzhi is sweet and mild in taste, slightly cold in nature, has the effects of invigorating the spleen and removing dampness, relieving cough and asthma, clearing heat and detoxification, and anti-tumor. Yunzhi polysaccharide is the main active ingredient of Yunzhi. It can activate immune cells, improve the immune function of the body, anti-tumor, lower blood fat and antibacterial. It is a safe and effective immune enhancer for humans or animals, and can be used as an additive for health food. [0003] At present, the extraction methods of Yunzhi polysaccharides mainly include w...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 刘巴宁郑安乔
Owner 中山安荞生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap