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Rapid propagation method of cyclobalanopsis glauca

A green and fast technology, applied in the field of plants, can solve the problems of high survival rate, low production cost and short growth cycle of tissue culture methods, and achieve the effects of short cycle, low pollution and large yield.

Inactive Publication Date: 2015-01-07
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For mastitis, there is currently no research on cultivation and planting. The tissue culture method has a high survival rate, low production cost, and a short growth cycle, which is conducive to large-scale promotion of planting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Freshly extracted Qinggang, cut into small sections of about 2cm with axillary buds or terminal buds, first soak in bleach for 3 minutes, remove surface stains with a brush, rinse with running water for 45 minutes, and treat with 400 μg / g benzyl ammonium blue solution on a clean bench for 20 minutes, Rinse 5-7 times with sterile water, and insert the sterilized Qinggang bud stem section into the culture medium formula is N 6 +6-BA1.0mg / L+NAA0.1mg / L induction medium for axillary bud induction culture, added sugar 30g / L, agar 6.5g / L, pH5.8, light 2500lx, temperature 26±1℃, induced Put the buds into the medium 1 / 2N 6 +IAA0.1mg / L+50mg / L tomato juice for proliferation culture, add sugar 30g / L, agar 6.5g / L, pH5.8, light 3000lx, temperature 26±1℃, put the clustered shoots cultured for rooting Medium 3 / 4N 6 +NAA0.05mg / L+80mg / L cysteine+sucrose 20g / L, agar 6.5g / L, pH5.8, light 4000lx, temperature 28°C, test-tube plantlets about 5cm long, taken out from the culture bottle, 0.5...

Embodiment 2

[0011] Freshly extracted Qinggang, cut into small sections of about 2cm with axillary buds or terminal buds, first soak in bleach for 3 minutes, remove surface stains with a brush, rinse with running water for 45 minutes, and treat with 400 μg / g benzyl ammonium blue solution on a clean bench for 20 minutes, Rinse 5-7 times with sterile water, and insert the sterilized Qinggang bud stem section into the culture medium formula is N 6 +6-BA1.0mg / L+NAA0.1mg / L induction medium for axillary bud induction culture, added sugar 30g / L, agar 6.5g / L, pH5.8, light 2500lx, temperature 26±1℃, induced Put the buds into the medium 1 / 2N 6 +IAA0.3mg / L+50mg / L tomato juice for proliferation culture, add sugar 30g / L, agar 6.5g / L, pH5.8, light 3000lx, temperature 26±1°C, put the clustered shoots cultivated for rooting Medium 3 / 4N 6 +NAA1.0mg / L+100mg / L cysteine+sucrose 20g / L, agar 6.5g / L, pH5.8, light 4000lx, temperature 28°C, test-tube plantlets about 5cm long, taken out from the culture bottle, ...

Embodiment 3

[0013] Freshly extracted Qinggang, cut into small sections of about 2cm with axillary buds or terminal buds, first soak in bleach for 3 minutes, remove surface stains with a brush, rinse with running water for 45 minutes, and treat with 400 μg / g benzyl ammonium blue solution on a clean bench for 20 minutes, Rinse 5-7 times with sterile water, and insert the sterilized Qinggang bud stem section into the culture medium formula is N 6 +6-BA1.0mg / L+NAA0.1mg / L induction medium for axillary bud induction culture, added sugar 30g / L, agar 6.5g / L, pH5.8, light 2500lx, temperature 26±1℃, induced Put the buds into the medium 1 / 2N 6 +IAA0.3mg / L+50mg / L tomato juice for proliferation culture, add sugar 30g / L, agar 6.5g / L, pH5.8, light 3000lx, temperature 26±1°C, put the clustered shoots cultivated for rooting Medium 3 / 4N 6 +NAA0.05mg / L+90mg / L cysteine+sucrose 20g / L, agar 6.5g / L, pH5.8, light 4000lx, temperature 28°C, test-tube plantlets about 5cm long, taken out from the culture bottle, ...

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Abstract

The invention provides a rapid propagation method of cyclobalanopsis glauca. The rapid propagation method comprises the steps of sterilization of a stem, induction of cluster buds, multiplication of cluster buds, rooting culture, acclimatization and transplanting, and the like. The cyclobalanopsis glauca prepared by the rapid propagation method is high in survival rate, good in quality, short in growth cycle and good in genetic stability, and can be popularized and planted in a large scale.

Description

technical field [0001] The invention relates to a rapid propagation method of Qinggang tissue culture, which belongs to the technical field of plants. Background technique [0002] Qinggang tree, Cyclobalanopsis glauca (Thunb.) Oerst , Fagaceae, also known as oak, acorn tree, Quercus qinggang, evergreen trees, subtropical tree species, up to 20 meters. The leaves are alternate, set on the top of branches, leathery, oval, obovate-elliptic, with short acuminate tips on the top of the leaves, wide wedges at the base of the leaves to form a circle, and teeth above the middle of the leaves. Female flowers in axils of several shoots. The shell is bowl-shaped, about 1 cm in diameter; the nut is ellipsoidal, 1.2-1.7 cm long, and 1 cm in diameter. It is one of the most widely distributed tree species in my country. North Korea, Japan, and India are also distributed. In the northern subtropical deciduous and evergreen broad-leaved mixed forest area south of the Yangtze River, it ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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