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Ultrasonic-assisted method for extracting polyphenols from redrlowered loropetalum leaves

A technology of safflower wood and polyphenols, which is applied in the directions of drug combination, pharmaceutical formula, plant raw material, etc., to achieve the effects of high efficiency, simple method and good effect.

Inactive Publication Date: 2015-01-07
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ultrasonic-assisted extraction technology has the advantages of simple and quick operation, time-saving, high efficiency, energy-saving, and little damage to active ingredients. At present, there is no research report on the use of ultrasonic-assisted extraction of polyphenols in Loropetalum safflower leaves

Method used

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  • Ultrasonic-assisted method for extracting polyphenols from redrlowered loropetalum leaves
  • Ultrasonic-assisted method for extracting polyphenols from redrlowered loropetalum leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) pulverizing the leaves of Loropetalum safflower into powder;

[0023] (2) Then add ethanol to the leaf powder of Loropetalum safflower, the ratio of solid to liquid is 1:10, put it into the ultrasonic cleaner, the ultrasonic power is 300W, the extraction temperature is 40°C, the extraction time is 60min, repeat the steps ( 2) extract 4 times, the extraction is completed, and the extracts are combined;

[0024] (3) Put the extract in step (2) into a refrigerated high-speed centrifuge for low-temperature high-speed centrifugation to obtain a supernatant containing polyphenols.

[0025] In step (1), the concentration of ethanol is 20%.

[0026] In step (3), the temperature of the low-temperature high-speed centrifugation is controlled below 5° C., and the centrifugation is performed at 10,000 r / min for 15 minutes.

[0027] In step (3), the supernatant was transferred to a brown volumetric flask wrapped with tinfoil and refrigerated at -20°C.

Embodiment 2

[0029] (1) pulverizing the leaves of Loropetalum safflower into powder;

[0030] (2) Then add ethanol to the leaf powder of Loropetalum safflower, the ratio of solid to liquid is 1:50, put it into the ultrasonic cleaner, the ultrasonic power is 800W, the extraction temperature is 80°C, the extraction time is 15min, repeat the steps ( 2) Extract once, and the extraction is completed to obtain the extract;

[0031] (3) Put the extract in step (2) into a refrigerated high-speed centrifuge for low-temperature high-speed centrifugation to obtain a supernatant containing polyphenols.

[0032] In step (1), the concentration of ethanol is 80%.

[0033] In step (3), the temperature of the low-temperature high-speed centrifugation is controlled below 5° C., and the centrifugation is performed at 10,000 r / min for 15 minutes.

[0034] In step (3), the supernatant was transferred to a brown volumetric flask wrapped with tinfoil and refrigerated at -20°C.

Embodiment 3

[0036] (1) pulverizing the leaves of Loropetalum safflower into powder;

[0037] (2) Then add ethanol to the leaf powder of Loropetalum safflower, the ratio of solid to liquid is 1:30, put it into the ultrasonic cleaner, the ultrasonic power is 600W, the extraction temperature is 65°C, the extraction time is 30min, repeat the steps ( 2) Extract 2 times, the extraction is completed, and the extracts are combined;

[0038] (3) Put the extract in step (2) into a refrigerated high-speed centrifuge for low-temperature high-speed centrifugation to obtain a supernatant containing polyphenols.

[0039] In step (1), the concentration of ethanol is 60%.

[0040] In step (3), the temperature of the low-temperature high-speed centrifugation is controlled below 5° C., and the centrifugation is performed at 10,000 r / min for 15 minutes.

[0041] In step (3), the supernatant was transferred to a brown volumetric flask wrapped with tinfoil and refrigerated at -20°C.

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Abstract

The invention discloses an ultrasonic-assisted method for extracting polyphenols from redrlowered loropetalum leaves. The method comprises the following steps: (1) crushing redrlowered loropetalum leaves into powder; (2) adding ethanol to the redrlowered loropetalum leaf powder, wherein the ratio of solid to liquid is 1 to (10-50), putting into an ultrasonic cleaner, repeating the step (2) and extracting for 1-4 times under the condition that the ultrasonic power is 300-800W, the extraction temperature is 40-80 DEG C and the extraction time is 15-60 minutes, after extraction is ended, mixing extracting solutions; and (3) putting the extracting solutions into a refrigeration high-speed centrifugal machine to carry out low-temperature high-speed centrifugation to obtain redrlowered loropetalum leaf extract. By the redrlowered loropetalum leaf extract disclosed by the invention, the activity of SOD enzyme and catalase in an aging model mice liver can be significantly improved, the MDA content is lowered, membrane lipid is prevented from being per-oxidized; the damage effects of reactive oxide species on cells are restrained; the redrlowered loropetalum leaf extract can be combined with various age-related effect factors to slow down cell apoptosis and senescence caused by inside and outside environment disorder of the organism; and the redrlowered loropetalum leaf extract has a relatively strong anti-aging effect.

Description

technical field [0001] The invention relates to the technical field of plant extraction methods, in particular to a method for extracting polyphenols from leaves of Loropetalum safflower assisted by ultrasonic waves. Background technique [0002] Loropetalum chinense Olive var. rubrum Yieh is a variant of L. chinense Olive in the family Hamamelidaceae. It is an evergreen shrub or small tree. It is a native wild resource in Hunan, with sporadic Distributed in Liuyang, Pingjiang, Liling, Hunan, and Hunan and Jiangxi, Pingxiang, Shangli District, Jiangxi. It is recorded in "Compendium of Materia Medica", "Plant Test of Plant Names and Facts", "Fujian Folk Medicine", and "Hunan Medicine Records" that the roots, leaves, flowers, and fruits of Prunus chinensis can be used as medicines, which have the functions of clearing away heat and detoxification, dredging channels and activating collaterals, Cough-relieving, anti-inflammatory, analgesic, hemostatic, stimulating the uterus, d...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P39/06A61K127/00
CPCA61K36/185A61K2236/333A61K2236/51
Inventor 朱丽娜于晓英陈仕贵陈淼芳
Owner HUNAN AGRICULTURAL UNIV
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