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Method for extracting rapamycin

A technology of rapamycin and concentrated solution, which is applied in the field of separation and purification, can solve problems such as poor concentration effect, and achieve the effects of reduced residue, high yield and improved efficiency

Active Publication Date: 2016-02-17
CHANGZHOU LANLING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0007] In addition, it can be seen from the above literature that multiple concentrations are required in the process of extracting rapamycin, and the commonly used concentration is rotary evaporation concentration or rising film falling film concentration. Dehydration, the above two concentration effects are not good

Method used

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Examples

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Effect test

Embodiment 1）

[0016] The method for extracting rapamycin of the present embodiment has the following steps:

[0017] ① Filter the fermentation broth (300L, titer 1.0g / L) containing rapamycin, extract the filter cake 3 times with acetone, then filter again, and concentrate the filtrate in vacuum to obtain the first concentrate.

[0018] ②The first concentrated solution obtained in step ① first flows through the pretreated AB-8 macroporous adsorption resin column at a volume of 10 times and a flow rate of 5BV / h, so that rapamycin is adsorbed on the macroporous resin. The pretreatment is to soak the AB-8 macroporous adsorption resin overnight with 50% ethanol by volume fraction to remove the remaining porogen and oligomers in the resin; then wash the resin 5 times with distilled water until no Ethanol smell; finally filter the resin to remove water and pack it into a column.

[0019] Then the macroporous adsorption resin column is carried out gradient elution, specifically as follows: first u...

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Abstract

The invention discloses a method for extracting rapamycin. The method for extracting the rapamycin comprises the following steps: (1) filtering a rapamycin-containing fermentation liquid, extracting a filter cake by an organic solvent, filtering again and concentrating a filtration solution to obtain a first concentration solution; (2) adsorbing the first concentration solution by a macro-porous adsorption resin column, then eluting the macro-porous adsorption resin column, concentrating the collected eluent to obtain a second concentration solution; (3) extracting and then concentrating the second concentration solution to obtain a third concentration solution; (4) carrying out column chromatography treatment on the third concentration solution, then eluting again, concentrating the collected eluent again to obtain a fourth concentration solution; and (5) crystallizing the fourth concentration solution again, then crystallizing again to obtain rapamycin. According to the method for extracting the rapamycin, the macro-porous adsorption resin is AB-8 macro-porous adsorption resin; the elution solvent is acetone, so that the high-content, high-purity and high-yield rapamycin can be finally obtained.

Description

technical field [0001] The invention belongs to the technical field of separation and purification, and in particular relates to a method for extracting rapamycin. Background technique [0002] Rapamycin, also known as sirolimus, is a macrolide antibiotic. It is a new type of immunosuppressant with good curative effect, low toxicity and no nephrotoxicity. It is extracted from the fermentation broth of Streptomyces hygroscopicus . [0003] Chinese patent document CN102070652A discloses a method for separating and extracting sirolimus from a fermented liquid, comprising filtering or centrifuging the sirolimus fermented liquid to obtain mycelia, and then extracting, decolorizing, and concentrating under reduced pressure to obtain sirolimus. The crude extract of sirolimus is then separated by high-speed countercurrent chromatography, and finally concentrated under reduced pressure and dried to obtain pure sirolimus. This method requires the use of an expensive high-speed count...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D498/18
CPCB01D15/185B01D15/265C07D498/18
Inventor 虞小平殷侃
Owner CHANGZHOU LANLING PHARMA
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