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Method for improving adversity resistance of plants

A plant, adversity technology, applied in the field of genetic technology and botany, can solve many problems such as

Inactive Publication Date: 2015-01-14
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, it has always been one of the difficulties in the research of plant genetic engineering to improve the stress resistance of forest trees such as salt tolerance and drought resistance through transgenic technology. At present, there are not many successful examples at home and abroad.

Method used

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  • Method for improving adversity resistance of plants
  • Method for improving adversity resistance of plants
  • Method for improving adversity resistance of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Example 1, Discovery and sequence structure analysis of PtCBL10A and PtCBL10B

[0106] The inventors found two CBL10 genes in the poplar genome, named PtCBL10A and PtCBL10B. The full-length cDNAs of these two genes were obtained by PCR method, and the related sequences were confirmed by further sequencing.

[0107] Through protein amino acid sequence comparison, the inventors found that the N-terminals of PtCBL10A (SEQ ID NO: 2) and PtCBL10B (SEQ ID NO: 4) have about 40 more amino acids than PtSOS3, which contains a putative span Membrane domains, and they also have four basic EF hand motifs, which are the same as SOS3, such as Figure 1-2 .

Embodiment 2

[0108] Embodiment 2, expression pattern research

[0109] The present inventors determined the expression patterns of PtCBL10A and PtCBL10B by RT-PCR semi-quantitative method. The experimental results show that the expression intensity of PtCBL10A is relatively uniform in various tissues and organs, and the expression intensity is slightly stronger in the terminal bud; while PtCBL10B is mainly expressed strongly in the tissues of the aerial part, and the expression level in the root is less, which is consistent with the expression of PtSOS3. The opposite is true ( image 3 A), and the experimental results in two different varieties of Populus trichocarpa and Populus Yinzhong are consistent ( image 3 B).

[0110] In order to further confirm and quantify this result, the inventors conducted a real-time PCR experiment and found that the expression level of PtCBL10A in the root and the aerial part is indeed similar; the expression level of PtCBL10B in the aerial part is nearly ...

Embodiment 3

[0113] Embodiment 3, complementary experiment

[0114]The present inventors expressed PtCBL10A and PtCBL10B driven by the constitutive promoter 35S in the background of the Arabidopsis mutant cbl10, and the tolerance of the transgenic plants to salt stress basically returned to the level of wild-type plants, while the corresponding constitutive expression PtSOS3 was completely unable to complement the salt-sensitive phenotype of the cbl10 mutant ( Figure 4 ).

[0115] This experiment shows that the PtCBL10A and PtCBL10B proteins of poplar can improve the salt tolerance of plants.

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Abstract

The invention relates to a method for improving the adversity resistance of plants. According to the method for improving the adversity resistance of the plants, a coding gene of CBL10 polypeptide of a poplar tree is transferred into the plants, further the salt resistance, thermal resistance and drought resistance of the plants are improved, growth of the plants is promoted, and biomass of the plants is improved. The method for improving the adversity resistance of the plants can be applied to improvement of plant variety.

Description

technical field [0001] The invention belongs to the fields of gene technology and botany, and more particularly relates to the application of calmodulin B subunit-like protein family genes in plants to improve stress resistance. Background technique [0002] Soil salinization is a worldwide resource and ecological problem, which seriously restricts the development of modern agriculture. Among all the arable land in our country, the area of ​​saline-alkali land is about 100 million mu, and the area of ​​medium and low-yield fields is as high as 1 billion mu. In addition, there are 300 million mu of saline-alkali wasteland for people to develop and utilize. The salt tolerance threshold of most crops and trees is lower than the salt concentration of 0.6%, while the salt content of large areas of saline-alkali land in my country is around 1.0%. With the rapid development of modern society, the population continues to expand, environmental pollution and ecological deterioration ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/84C07K14/415A01H5/00
Inventor 张洪霞唐仁杰杨阳
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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