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Electrochemical biosensor and application thereof

A biosensor and electrochemical technology, applied in the field of bioelectrochemical sensors, can solve problems such as unsuitable for on-site operation, long analysis time, poor stability and reproducibility, achieve good stability and reproducibility, and avoid poisoning events , the effect of wide detection range

Active Publication Date: 2015-01-14
SUZHOU CHIEN SHIUNG INST OF TECH
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Problems solved by technology

The disadvantages of using high-performance liquid chromatography / mass spectrometry (HPLC-MS) or gas chromatography / mass spectrometry (GC-MS) detection are: long analysis time, expensive equipment, professional and technical personnel are required to operate, and it is not suitable for on-site operation
However, the existing sensors have a narrow detection range, poor stability and reproducibility, and are not suitable for real sample detection of vegetable organophosphorus pesticide methamidophos residues

Method used

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  • Electrochemical biosensor and application thereof
  • Electrochemical biosensor and application thereof
  • Electrochemical biosensor and application thereof

Examples

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Embodiment Construction

[0022] The present invention will be described in detail below through specific examples but not intended to limit the present invention.

[0023] The preparation method and application steps of the electrochemical biosensor of the present invention are as follows:

[0024] (1) Build an electrochemical biosensor:

[0025] A) Synthesis of monodisperse gold nanofilms at the organic phase-aqueous phase interface by liquid-liquid interface method:

[0026] First 1.9mg PPh 3 Added to 25μL 0.24mol / L HAuCl4 to react to form Au(PPh 3 ) Cl, which was then transferred to 5.0 mL of toluene to form an organic phase solution; secondly, 0.8 mg of CTAB (cetyltrimethylammonium bromide) and 2 mg of NaOH were dissolved in 8 mL of H 2 Form an aqueous phase solution in O, and add 25 μL N 2 h 4 ·H 2 O was added to the aqueous phase as a reducing agent; then the organic phase was slowly added to the aqueous phase. When the interface appears purple, it indicates that the reaction has started....

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Abstract

The invention discloses an electrochemical biosensor. A monodisperse gold nano film is synthesized by adopting a liquid-liquid interface method and used for modifying the surface of an ITO electrode, and the surface of the ITO electrode is further modified by acetylcholin esterase and chitosan to obtain a composite electrochemical biosensor. The electrochemical biosensor can be used for detecting methamidophos in organophosphorous pesticide, is wide in detection range, low in detection limit and high in stability and repeatability and can be used for detecting the residual real methamidophos sample of organophosphorous pesticide for vegetables.

Description

technical field [0001] The invention belongs to the technical field of bioelectrochemical sensors, and in particular relates to an electrochemical biosensor and its application. Background technique [0002] China Agricultural Instrument Network once reported on the investigation of organophosphorus pesticides in vegetables in Shaanxi Province, and found that methamidophos ranked in the top five in detection rate and exceeding standard rate, which shows that the problem of pesticide residues in vegetables is still very serious in my country. The disadvantages of using high-performance liquid chromatography / mass spectrometry (HPLC-MS) or gas chromatography / mass spectrometry (GC-MS) detection are: long analysis time, expensive equipment, professional and technical personnel are required to operate, and it is not suitable for on-site operation. Electrochemical detection is fast, sensitive, and simple. Therefore, electrochemical biosensors constructed by using methamidophos to i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 苗向阳郁惠珍戴志晖
Owner SUZHOU CHIEN SHIUNG INST OF TECH
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