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A kind of cultivation method for increasing Flammulina velutipes polysaccharide content

A technology of Flammulina velutipes polysaccharides and cultivation methods, applied in the cultivation field of increasing the polysaccharide content of Flammulina velutipes, achieving the effects of increasing polysaccharide content, low energy consumption, and less cost increase

Active Publication Date: 2016-06-29
拜肯生物科技(上海)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, some progress has been made in the research on fungal polysaccharides, mainly focusing on how to extract fungal polysaccharides and how to improve the extraction methods of polysaccharides, but little is known about how to improve the cultivation of Flammulina velutipes polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Select the following mother seed medium: 200 grams of potato, 20 grams of sucrose, 2 grams of peptone, vitamin B 1 0.5 g, MgSO 4 0.5g, KH 2 PO 4 2 grams, 1000 ml of water, natural pH;

[0029] (2) Select cultivar culture medium: bagasse 75%, rice bran or wheat bran 22%, sucrose 1%, gypsum 1%, soybean powder 1%; Flammulina velutipes bacterial strain is cultivated in described cultivar culture medium 5~8 Take it out two days later, and then induce it for 10 minutes at a temperature of 45°C;

[0030] (3) Continue to cultivate: place the Flammulina velutipes culture medium through the above-mentioned treatment in an air bath shaker, cultivate at a constant temperature of 25° C., and cultivate for 3 days at 180 rpm;

[0031] (4) Set up a scaffold and cover it with red or purple film;

[0032] (5) Selecting a suitable nutrient substrate for cultivation, the nutrient substrate is prepared from the following components by weight, 100 parts of rice straw, 70 parts of co...

Embodiment 2

[0037] (1) Select the following mother seed medium: 200 grams of potato, 20 grams of sucrose, 2 grams of peptone, vitamin B 1 0.5 g, MgSO 4 0.5g, KH 2 PO 4 2 grams, 1000 ml of water, natural pH;

[0038] (2) Select cultivar culture medium: bagasse 75%, rice bran or wheat bran 22%, sucrose 1%, gypsum 1%, soybean powder 1%; Flammulina velutipes bacterial strain is cultivated in described cultivar culture medium for 6 days and then taken out , and then induced at a temperature of 50°C for 8 minutes;

[0039] (3) Continue to cultivate: place the Flammulina velutipes culture medium through the above-mentioned treatment in an air bath shaker, cultivate at a constant temperature of 25°C, 180 rpm, and cultivate for 4 days;

[0040] (4) Set up a scaffold and cover it with red or purple film;

[0041] (5) Selecting a suitable nutrient substrate for cultivation, the nutrient substrate is formulated from the following components by weight, 150 parts of rice straw, 90 parts of cow dun...

Embodiment 3

[0046] (1) Select the following mother seed medium: 200 grams of potato, 20 grams of sucrose, 2 grams of peptone, vitamin B 1 0.5 g, MgSO 4 0.5g, KH 2 PO 4 2 grams, 1000 ml of water, natural pH;

[0047] (2) Select cultivar medium: bagasse 75%, rice bran or wheat bran 22%, sucrose 1%, gypsum 1%, soybean powder 1%; Flammulina velutipes bacterial strain is cultivated in described cultivar medium for 8 days and then taken out , and then induced for 6 minutes at a temperature of 55°C;

[0048] (3) Continue to cultivate: place the Flammulina velutipes culture medium through the above-mentioned treatment in an air bath shaker, cultivate at a constant temperature of 25° C., and cultivate for 3 days at 180 rpm;

[0049] (4) Set up a scaffold and cover it with red or purple film;

[0050] (5) Selecting a suitable nutrient substrate for cultivation, the nutrient substrate is prepared from the following components by weight, 200 parts of rice straw, 150 parts of cow dung, 80 parts o...

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PUM

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Abstract

The invention discloses a cultivation method for improving the polysaccharide content of flammulina velutipes. The method comprises various measures of mother strain culture medium selection, cultivated specie culture medium selection, matrix selection, thin film color selection, fertilizer and water management, control over the temperature difference between the day and the night and carbon dioxide in a greenhouse and the like. At last, the polysaccharide content of the flammulina velutipes treated by the method can be improved by 16% maximally.

Description

technical field [0001] The invention relates to a cultivation method, in particular to a cultivation method for increasing the polysaccharide content of Flammulina velutipes. Background technique [0002] In recent years, many polysaccharides have been found in fungal research not only have anti-tumor and high immune effects, but also have good pharmacological and clinical value, and have been developed into clinical drugs. Modern scientific research has proved that edible fungus polysaccharides can enhance the body's immunity and cellular immunity; it can excite the reticuloendothelial system and protect the animal liver; it has broad-spectrum anti-virus, anti-tumor immunity and Solubilization of cholesterol. At present, no suitable agent has been found for the treatment of plant virus diseases in the world. Therefore, looking for immune enhancers is one of the directions for developing new drugs today, and fungal polysaccharides are ideal new immune agents. It is non-tox...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 韦俊
Owner 拜肯生物科技(上海)股份有限公司