Method for detecting multiple fungal toxins in egg

A technology for mycotoxins and eggs, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of high experimental cost and cumbersome operation process, and achieve the effects of reducing pollution, improving extraction efficiency and reducing system errors.

Inactive Publication Date: 2015-01-21
UNIV OF SHANGHAI FOR SCI & TECH +1
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Problems solved by technology

However, the general extraction process requires the use of solid-phase extraction columns, which is cumbersome and expensive to operate.

Method used

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  • Method for detecting multiple fungal toxins in egg
  • Method for detecting multiple fungal toxins in egg
  • Method for detecting multiple fungal toxins in egg

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Experimental program
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Embodiment Construction

[0033] The method for detecting multiple mycotoxins in eggs of the present invention is as follows:

[0034] 1 Experimental method

[0035] 1.1 Sample pretreatment method

[0036] Step (1) Take a fresh egg and crack the shell, take the egg yolk and egg white, stir evenly to form an egg liquid, take 1g of the egg liquid in a 50mL polypropylene centrifuge tube, add 4mL ultrapure water, use a vortex instrument (brand: WIGGENS , model Vortex3000) vortex for 2 minutes, let it infiltrate for 10 minutes;

[0037] Step (2) Add 5 mL of acetonitrile solution containing 1% formic acid, vortex for 2 min, tighten the cap, place horizontally on a mechanical shaker and extract by shaking at a speed of 80 rpm for 30 min;

[0038] Step (3) Then add 2g (± 0.5g) anhydrous magnesium sulfate and 0.5g (± 0.01g) sodium chloride to the centrifuge tube in turn. After adding salt, immediately tighten the lid and shake to prevent salt from agglomerating, vortex 3min to induce toxin partition;

[003...

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Abstract

The invention provides a method for detecting multiple fungal toxins in an egg. The method comprises the following steps: step 1, sequentially adding water and an organic extracting agent into a raw and fresh egg sample which is evenly stirred, whirling and then carrying out mechanical oscillation for certain time; step 2, adding a certain amount of salt into the egg sample after the oscillation extraction, whirling, centrifuging to obtain a supernatant, and drying the supernatant; and step 3, redissolving the dried sample with a mobile phase applied to liquid chromatography tandem mass spectrometry, then filtering and carrying out qualitative or quantitative detection on the fungal toxins by virtue of a liquid chromatograph / mass spectrometer. According to the method, few pretreatment steps are carried out on the sample, so that the time consumption of a sample pretreatment phase in a whole food analysis experiment is greatly reduced, the loss of a target compound in an operating process is lowered, system errors are greatly reduced and the extraction efficiency of the target compound is improved; and all adopted reagents are very cheap and are less in usage amount, so that the experiment cost is lowered and the environmental pollution is reduced.

Description

technical field [0001] The invention relates to a method for detecting multiple mycotoxins in eggs, belonging to the field of food detection. Background technique [0002] Analytical chemistry is a science that studies the characterization and measurement of the chemical composition of substances. Its main task is to identify the chemical composition and structure of substances and measure the content of related components. It is an indispensable and effective means of quality, and it is one of the fastest-growing disciplines in recent years. Two prominent problems in food analysis: one is sample pretreatment technology; the other is analysis and detection technology. Sample pretreatment is the bottleneck of analytical chemistry and one of the difficulties and hot issues in analytical chemistry research. It refers to the process of sample preparation and extraction, purification and concentration of the components to be measured in the sample. Food analysis differs from ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02
Inventor 徐斐朱闰月宋素泉武爱波赵志勇曹慧刘娜
Owner UNIV OF SHANGHAI FOR SCI & TECH
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