Sputum collection method for detecting 16SrRNA gene sequence of respiratory tract bacteria

Abstract

The invention discloses a sputum collection method for detecting the 16SrRNA gene sequence of respiratory tract bacteria. The sputum collection method comprises the following steps: step one, measuring the forced vital capacity FEV1 in the first second of the foundation; step two, absorbing 200 mu. G of salbutamol aerosol; step three, blowing the nose and rinsing the mouth with 0.9% of sterilization normal saline water; step four, carrying out atomization induction with 3% of sterilization saline water for 10 min, stopping atomization, rinsing the mouth for three times with 0.9% of sterilization normal saline water, and holding the breath to cough up deep sputum, so that the sputum is colleted in a sterile culture dish; step five, carrying out atomization with 4% and 5% sterilization saline water respectively to repeat the above steps, and collecting the deep sputum; step six, separating sputum bolt collected in the step four and the step five and preserving the sputum bolt at a low temperature. The sputum collection method for detecting the 16SrRNA gene sequence of respiratory tract bacteria provided by the invention is low in possibility to contaminate an oral cavity, and is simple and convenient.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for collecting sputum for detecting the sequence of the 16S rRNA gene of respiratory tract bacteria. Background technique [0002] The study of respiratory tract microbiome is a relatively new field. 16S rRNA gene sequence analysis is a molecular detection technology commonly used to identify bacterial species, and has been widely used in the study of environmental and clinical specimens. The study of respiratory tract microbiome is a A relatively new field. The 16S rRNA gene is the DNA sequence corresponding to the coding rRNA on the bacterial chromosome, which exists in the chromosomal genome of all bacteria. The 16S rRNA sequence information obtained by needle hybridization is compared with the sequence data in the 16S rRNA database or other data to determine its position in the evolutionary tree, thereby identifying the possible microbial species in the sample. Therefore...

AI Technical Summary

Problems solved by technology

Coughing out sputum directly after gargling and placing it in a sterile culture bottle is a common collection method for clinical sputum collection and bacterial culture at present. Defects such as more epithelial cells

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