Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of preparation method of antitumor hydrogel

A hydrogel and anti-tumor technology, applied in the field of biomedical materials, can solve the problems of clinical application limitations, shortened release cycle, and excessive release, etc., to achieve efficient control of drug release speed, enhance anti-tumor effect, and prolong release time Effect

Inactive Publication Date: 2017-02-15
JIANGSU UNIV OF SCI & TECH
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in addition to causing myelosuppression, gastrointestinal toxicity and hair loss, the drug can still cause severe cardiotoxicity, which limits its clinical application to a certain extent.
[0005] Doxorubicin (DOX) hydrogel is usually based on gelatin, and DOX is loaded into the hydrogel in a simple and dispersed manner, but there is a problem that the release rate is difficult to control, usually the release is too fast and the release period is shortened , especially when the loading amount is large, the rapid release will cause the plasma concentration to rise too fast, and the toxicity to the heart will increase

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of antitumor hydrogel
  • A kind of preparation method of antitumor hydrogel
  • A kind of preparation method of antitumor hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Mix the graphene oxide solution loaded with doxorubicin (the content of doxorubicin is 4mg / ml, the concentration of graphene oxide is 2mg / ml) and 7.5% (W / V) sericin solution in the dark Obtain mixed solution, the volume ratio of the two is 1:5;

[0021] Mix the above mixture with 10% (W / V) gelatin solution, the volume ratio of the two is 1.5:1, then add glutaraldehyde, add 0.05g of 25% (W / V) glutarin for every 1g of sericin The aldehyde was reacted at 40° C. for 20 minutes to obtain a graphene oxide-doxorubicin-sericin-gelatin hydrogel, which is the anti-tumor hydrogel of the present invention.

Embodiment 2

[0023] A solution of graphene oxide loaded with doxorubicin (the content of doxorubicin is 2.5 mg / ml, and the concentration of graphene oxide is 1.5 mg / ml) and 0.5% (W / V) sericin solution were prepared under dark conditions. Mix evenly to obtain a mixed solution, and the volume ratio of the two is 1:3.

[0024] Mix the above mixture with 5% (W / V) gelatin solution, the volume ratio of the two is 1:1, then add glutaraldehyde, add 0.15g of 25% (W / V) glutaraldehyde per 1g of sericin Aldehydes were reacted at 30° C. for 4 hours to obtain graphene oxide-doxorubicin-sericin-gelatin hydrogel, which is the anti-tumor hydrogel of the present invention.

Embodiment 3

[0026] A solution of doxorubicin-loaded graphene oxide (the content of doxorubicin is 1 mg / ml and the concentration of graphene oxide is 1 mg / ml) was mixed with a 10% (W / V) sericin solution in the dark Obtain the mixed liquid evenly, and the volume ratio of the two is 1:2.

[0027] Mix the above mixture with 7.5% (W / V) gelatin solution, the volume ratio of the two is 0.5:1, then add glutaraldehyde, add 0.25g of 25% (W / V) glutaraldehyde per 1g of sericin The dialdehyde was reacted at 20° C. for 12 hours to obtain graphene oxide-doxorubicin-sericin-gelatin hydrogel, which is the anti-tumor hydrogel of the present invention.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing anti-tumor hydrogel. According to the method, sericin protein and gelatin are adopted as a matrix, adriamycin-loaded graphene oxide is dispersed in the matrix, glutaraldehyde is used as a crosslinking agent to promote crosslinking reactions between sericin proteins and between sericin protein and graphene oxide, gelatin molecules interpenetrate among the sericin protein and graphene oxide, and the anti-tumor hydrogel is finally formed. By adopting the anti-tumor hydrogel prepared by the method, on the one hand, the drug-release speed can be effectively regulated by regulating the concentration of sericin protein in the hydrogel, on the other hand, the drug load capacity of the hydrogel can be remarkably increased since the surface area of the graphene oxide is extremely large, and the effects of enhancing the anti-tumor effect and relieving pains on patients caused by multiple drug taking times can be achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a preparation method of an anti-tumor hydrogel. Background technique [0002] Hydrogel is a common gel, which is a three-dimensional network structure formed by cross-linking through covalent bonds, van der Waals forces, hydrogen bonds, etc. It is a polymer that can swell but not dissolve in water, and has good water absorption. A kind of functional polymer material integrating water absorption, water retention and slow release. A part of hydrophobic groups and hydrophilic residues are introduced into the water-soluble polymer with a network crosslinked structure, and the hydrophilic residues combine with water molecules to connect the water molecules inside the network, while the hydrophobic residues swell with water. polymer. Any water-soluble or hydrophilic polymer can be cross-linked into a polymer network system in a certain way (physical or chemic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K31/704A61K47/42A61K47/04A61P35/00
Inventor 颜辉郭成蔡豪江明珠贾俊强吴琼英
Owner JIANGSU UNIV OF SCI & TECH