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Extraction method of deer spleen extract and application of deer spleen extract in medicine for improving immunity

An extract and immunity technology, which is applied to the invention discloses deer, the application of deer spleen extract in immunity-improving medicines, the field of deer spleen extract extraction, and achieves the improvement of the extraction rate of polypeptide and nucleic acid and the enhancement of immunity. Effect

Active Publication Date: 2015-01-28
吉林省东鳌鹿业科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]With the development of biotechnology, the extraction method of transfer factor has been gradually improved. There is a patent "a deer spleen extract preparation and its preparation method" patent number ZL201210171730.9 , the deer spleen is extracted by adding pepsin, trypsin or neutral protease for enzymolysis, the extraction rate of nucleic acid in the extract is still extremely low, because the protease selected can only enzymolyze part of the protein in deer spleen, the ability of enzymolysis Limited, the extraction rate of effective peptides extracted is also low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] . Weigh 200g of deer spleen, remove the surface connective tissue;

[0023] . Add 400ml of normal saline, and grind for 5 minutes with a high-speed shearing machine such as a colloid mill;

[0024] .Heat the deer spleen suspension to 70°C, adjust the pH to 6.0, add 4.8g of papain, 2.4g of bromelain and 5.0g of 5'-phosphodiesterase for enzymolysis and incubate for 2 hours;

[0025] . Centrifuge the suspension under 4000g centrifugal force for 10 minutes to take the supernatant, perform ultrafiltration with a 10KD ultrafiltration membrane to obtain an ultrafiltrate, and spray dry to obtain the deer spleen extract powder with the function of improving immunity. The extraction rate of peptides and nucleic acids was 12.67% and 1.79%.

Embodiment 2

[0027] . Weigh 200g of deer spleen, remove the surface connective tissue;

[0028] . Add 600ml of normal saline, and grind for 2 minutes with a high-speed shearing machine such as a colloid mill;

[0029] .Heat the deer spleen suspension to 65°C, adjust the pH value to 5.5, add 2.5g of papain, 2.5g of bromelain and 5.0g of 5'-phosphodiesterase for enzymatic hydrolysis and incubate for 2 hours;

[0030] . Centrifuge the suspension under 4000g centrifugal force for 10 minutes to take the supernatant, perform ultrafiltration with a 10KD ultrafiltration membrane to obtain an ultrafiltrate, and spray dry to obtain the deer spleen extract powder with the function of improving immunity. The extraction rate of peptides and nucleic acids was 12.32% and 1.57%.

Embodiment 3

[0032] . Weigh 200g of deer spleen, remove the surface connective tissue;

[0033] . Add 650ml of normal saline, and grind for 3 minutes with a high-speed shearing machine such as a colloid mill;

[0034] .Heat the deer spleen suspension to 65°C, adjust the pH to 5, add 2.7g of papain, 2.1g of bromelain and 5.1g of 5'-phosphodiesterase for enzymolysis and incubate for 3h;

[0035] . Centrifuge the suspension under 4000g centrifugal force for 10 minutes to take the supernatant, perform ultrafiltration with a 10KD ultrafiltration membrane to obtain an ultrafiltrate, and spray dry to obtain the deer spleen extract powder with the function of improving immunity. The extraction rate of peptides and nucleic acids was 12.13% and 1.68%.

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PUM

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Abstract

The invention discloses an extraction method of deer spleen extract and application of the deer spleen extract in medicine for improving immunity. Deer spleen is subjected to enzymolysis by adopting a compound enzyme prepared from papain, bromelain and 5'-phosphodiesterase; the extraction method has a synergistic effect; and compared with a method of adopting a single enzyme to extract, the method improves the polypeptide extraction ratio by 90 percent and improves the nucleic acid extraction ratio by 75 percent. The extraction method has industrial production value.

Description

Technical field: [0001] The invention discloses a deer spleen extract extraction method, and further provides the application of the deer spleen extract in medicines for improving immunity, which belongs to the technical field of biopharmaceuticals. technical background: [0002] Deer spleen is the spleen of deer or red deer. Chinese medical scientists in the past dynasties believe that the deer tail fairy beast, the thing with pure yang and long life, is beneficial to people all over the body. Deer products have extremely high medicinal value and health effects. Small molecules such as peptides or nucleic acids extracted from the spleen, also known as transfer factors, have the functions of reducing or eliminating the toxic and side effects caused by chemotherapy and radiotherapy in cancer patients, and are mainly used in the field of biomedicine. [0003] Transfer factor is a kind of dialyzable small molecular polypeptide and nucleotide complex released by T lymphocyte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/26A61P37/04A23L1/29A23L1/305A23L33/18
CPCA23L33/00A23L33/18A23V2200/324A23V2250/204A23V2300/38A61K9/14A61K35/26
Inventor 滕利荣彭小君蔡广胜贾东旭权宇彤孟庆繁逯家辉王迪滕乐生王贞佐谢晶程瑛琨
Owner 吉林省东鳌鹿业科技开发有限公司