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Method for producing glyceric acid catalyzed by whole cells of Acetobacter tropicalis

A technology of Acetobacter tropicalis and glyceric acid, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of complex fermentation products, difficult product extraction, and high extraction costs, and achieve simple product composition and easy purification. , the effect of reducing production costs

Active Publication Date: 2017-11-07
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, only Professor Habe of Japan has published relevant results on the production of glyceric acid by microorganisms (Hiroshi Habe, etc. 2009), and there is no report in China
At the same time, although glyceric acid can be obtained through traditional fermentation methods, there are complex fermentation products and many by-products, which lead to difficulties in product extraction and high extraction costs

Method used

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  • Method for producing glyceric acid catalyzed by whole cells of Acetobacter tropicalis
  • Method for producing glyceric acid catalyzed by whole cells of Acetobacter tropicalis
  • Method for producing glyceric acid catalyzed by whole cells of Acetobacter tropicalis

Examples

Experimental program
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Effect test

Embodiment 1

[0026] 1) Primary shake flask seed culture: Inoculate the glyceric acid-producing bacteria Acetobacter tropicalis CHM061701 at an inoculum amount of 1% in a shake flask containing a primary seed medium, and culture at 30° C. for 24 hours at 200 rpm. The composition of the medium is: glucose 5g / L, yeast powder 5g / L, peptone 5g / L, MgSO 4 ·7H 2 O1g / L, pH6.5.

[0027] 2) Second-stage shake flask seeds: inoculate the first-stage shake flask seeds of step 1) with a 5% inoculum amount into the shake flasks containing the second-stage seed medium, and cultivate them at 30° C. for 24 hours at 200 rpm. The composition of the medium is: glucose 5g / L, yeast powder 5g / L, peptone 5g / L, MgSO 4 ·7H 2 O1g / L, pH6.5.

[0028] 3) Production of glyceric acid by fermentation: inoculate the second stage shake flask seed in step 2) with an inoculum amount of 5% in a shake flask containing a fermentation medium, and culture at 30° C. for 96 hours at 200 rpm. The medium composition is: glycerol 15...

Embodiment 2

[0033] 1) Primary shake flask seed culture: the glyceric acid producing bacteria Acetobacter tropicalis CHM061701 stored in glycerol tubes was inoculated into a shake flask containing primary seed medium at an inoculum amount of 1%, and cultured at 30° C. and 200 rpm for 24 hours. The composition of the medium is: glucose 5g / L, yeast powder 5g / L, peptone 5g / L, MgSO 4 ·7H 2 O1g / L, pH6.5 before sterilization.

[0034] 2) Second-stage shake flask seeds: Inoculate the first-stage shake flask seeds of step 1) with an inoculum amount of 5% in a shake flask containing a second-stage seed medium, and culture at 30° C. for 24 hours at 200 rpm. The composition of the medium is: glucose 5g / L, yeast powder 5g / L, peptone 5g / L, MgSO 4 ·7H 2 O1g / L, pH6.5 before sterilization.

[0035] 3) Bacterial cell enrichment culture: inoculate the second-stage shake flask seeds in step 2) with an inoculum amount of 5% into a shake flask containing a bacterial cell enrichment medium, and culture at 3...

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Abstract

The invention discloses a method for catalyzing the production of glyceric acid by whole cells of Acetobacter tropicalis, relating to a production method of glyceric acid. 1) Primary shake flask seed culture: inoculate the glyceric acid producing bacterium Acetobacter tropicalis (Acetobacter tropicalis) CHM061701 in a shake flask equipped with a primary seed medium; The seeds cultivated by the first level shake flask seeds are inoculated in the shake flasks with secondary seed culture medium; Cultivate in shake flasks with culture medium; 4) Bacteria collection: take step 3) and centrifuge the cultured bacteria liquid, wash, collect the bacteria, then add glycerol aqueous solution, and adjust the initial pH to 5-8; 5) Catalytic conversion of whole cells : the aqueous glycerol solution containing thalline obtained in step 4) is catalytically converted in a shake flask to obtain glyceric acid.

Description

technical field [0001] The invention relates to a method for producing glyceric acid, in particular to a method for catalyzing the production of glyceric acid by whole cells of Acetobacter tropicalis. Background technique [0002] Glyceric acid was originally found in some plants, such as peanuts, artichoke leaves, tomatoes, bananas, apples, broad beans, grapes, etc. However, the enantiomeric composition and concentration of glyceric acid contained therein are still unknown. In animals, glyceric acid can also be detected in the liver of animals such as horses, cattle, pigs, mice, rabbits, etc., and mainly exists in the form of D-glyceric acid, which is the precursor of serine synthesis (Akira Ichihara, David M.Greenberg. 1957). At the same time, D-glyceric acid was also detected in the human body, which is an intermediate metabolite of fructose decomposition. [0003] D-glyceric acid present in the human body has the function of promoting the catabolism of ethanol and acet...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/42C12R1/02
CPCC12P7/42C12N1/205C12R2001/02
Inventor 卢英华蒲洋陈慧敏王宝贝敬科举陈翠雪
Owner XIAMEN UNIV