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A gold nanoparticle biosensor for detecting serum creatinine and its preparation method

A technology of gold nanoparticles and biosensors, which is applied in the fields of analyzing materials through chemical reactions, analyzing materials through observing the influence of chemical indicators, fluorescence/phosphorescence, etc., which can solve complex processing, time-consuming, and poor specificity. and other problems, to achieve the effect of simple operation, simple and convenient operation, and low cost

Active Publication Date: 2017-01-11
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have shown certain sensitivity and selectivity in the detection of serum creatinine, there are still many defects, such as: complex experimental operation and sample pretreatment, time-consuming, poor specificity, expensive instruments, etc.

Method used

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  • A gold nanoparticle biosensor for detecting serum creatinine and its preparation method
  • A gold nanoparticle biosensor for detecting serum creatinine and its preparation method
  • A gold nanoparticle biosensor for detecting serum creatinine and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0048] 1. Preparation of gold nanoparticles colloidal solution in a specific particle size range

[0049] Take 2.9 mL, 10 mmol / L of chloroauric acid (HAuCl 4 • 4H 2 O), dilute to the mark with deionized water in a 100 mL volumetric flask, boil, then add 3.5 mL, 1% (wt%) trisodium citrate solution, continue to stir and boil, after 10 min, stop heating, stir for about 20 min until the solution was naturally cooled to room temperature. The prepared AuNPs solution was stored at 4°C. The absorbance of the finally obtained gold nanoparticle solution was detected at 521 nm by an ultraviolet-visible spectrophotometer, thereby quantitatively determining the concentration of gold nanoparticles. When the absorbance of gold nanoparticles at 521 nm is 1.000, its concentration is recorded as 1×, and the concentration of gold nanoparticles is about 15nM (measured and calculated by ultraviolet spectrophotometer). pass image 3 From the TEM images, it can be deduced that the particle size...

Embodiment 2

[0057] 1. Protection of gold nanoparticles by different anti-aggregation agents

[0058] In order to protect the gold nanoparticles, the increase of RLS and the change of the color of AuNPs solution have a more gradient change to the change of serum creatinine concentration, so as to expand the range of creatinine colorimetric detection and quantitative analysis. The inventors tested various The protective effects of amino acids and GSH on gold nanoparticles, the test results are as follows Figure 5 and Figure 9 shown. Figure 5 The abscissa 1 represents GSH (0.2 μM); the abscissas 2 to 12 represent alanine, leucine, phenylalanine, glycine, arginine, lysine, tyrosine, tryptophan, threonine, respectively. amino acid, valine, histidine, the concentration is 1μM, by Figure 5 It can be seen that the concentration of fixed serum creatinine is 100 μM. It can be observed that after adding GSH and various amino acids, only the reaction system with GSH has an obvious ΔI RLS sign...

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Abstract

The invention discloses a gold nanoparticle (AuNPs) biological sensor for detecting serum creatinine and a preparation method of the gold nanoparticle biological sensor. The preparation method comprises the steps of (1) preparing an AuNPs solution; (2) adding a GSH solution serving as an anti-aggregating agent for uniform mixing, and performing surface protection on the AuNPs; and (3) respectively adding standard serum creatinine solutions with different concentrations, and performing colorimetric and quantitative detection by using a fluorospectro photometer to obtain a relation curve between the serum creatinine concentration and the resonance light scattering (RLS) intensity so as to obtain the gold nanoparticle biological sensor. GSH can form a protection layer on the surface of AuNPs, so that the aggregation degree of AuNPs, which is caused by the adding of the serum creatinine is reduced; under proper GSH concentration, the serum creatinine solutions with different concentrations are added, so that an AuNPs solution system shows a gradual color change phenomenon; meanwhile, by the use of the relation between an AuNPs dispersing state or the aggregating degree and the RLS intensity, quantitative detection on the serum creatinine can be realized. The method for visual measurement and RLS intensity detection on the serum creatinine has the advantages of simplicity, quickness, high practicability and the like.

Description

technical field [0001] The invention relates to the fields of analytical chemistry and nanometer material spectrum analysis, in particular to a gold nanoparticle biosensor for detecting serum creatinine and a preparation method thereof. Background technique [0002] In recent years, gold nanoparticles (AuNPs), as a biochemical nanosensing probe, have attracted more and more attention from researchers due to their advantages such as simplicity, rapidity, easy synthesis, high specific surface area and unique optical properties. At the same time, the dispersed state (wine red) or aggregated state (blue) of gold nanoparticles can be used as an important basis for colorimetric analysis. Colorimetric analysis is a quantitative analysis method that began in the 1930s and 1940s. It determines the content of the component to be tested by comparing or measuring the color depth of the colored substance solution. Choosing an appropriate color reaction and controlling the appropriate re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/78
Inventor 高文华黄晓鹏陈耀文张嘉宏陈佳阳陈图锋张晓珊
Owner SHANTOU UNIV
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