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Microarray substrate, microarray, microfluidic system and methods for preparing the same

A microfluidic system and microarray technology, applied in the direction of microbial library, chemical library, combinatorial chemistry, etc., can solve problems such as high cost

Active Publication Date: 2015-02-11
THE CHINESE UNIVERSITY OF HONG KONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most methods require expensive chemical reagents and strict control conditions, so specially trained personnel are usually required to operate, and the accompanying high cost

Method used

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  • Microarray substrate, microarray, microfluidic system and methods for preparing the same
  • Microarray substrate, microarray, microfluidic system and methods for preparing the same
  • Microarray substrate, microarray, microfluidic system and methods for preparing the same

Examples

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preparation example Construction

[0064] Fabrication of multilayer microfluidic loading devices

[0065] A multilayer microfluidic loading device was prepared to form polydopamine microspots on a fluoropolymer membrane. Microfluidic systems can also be used to deliver chemical or biochemical agents to the fluoropolymer substrates disclosed herein. The terms "microfluidic loading device" and "microfluidic system" are used interchangeably herein.

[0066] Microfluidic systems can be fabricated using a variety of materials such as polydimethylsiloxane (PDMS), polystyrene, polycarbonate, glass, and silicon wafers. For example, microfluidic systems are made of polydimethylsiloxane (PDMS). PDMS is a commonly used material for microfluidic chips 29 , PDMS is known to be gas and water permeable 32 . As an example, methods of fabricating microfluidic systems include the use of soft lithography 30 .

[0067] In one embodiment, the microfluidic system comprises or consists of two layers with different patterns. T...

Embodiment 1

[0092] Example 1: Preparation of PDMS microfluidic loading device

[0093]The PDMS microfluidic device consists of two PDMS layers with different patterns, by soft lithography using Siloxane 184 Silicone Elastomer kit (Dow Corning) to produce. The kit contains PDMS precursor and curing agent.

[0094] By casting PDMS precursors, liquid siloxane monomers ( 184, Dow Corning) and a mixture of curing agent (5:1) to make the upper PDMS layer. The silicon master is patterned using photolithography. The silicon master serves as a mold and contains a relief structure ( figure 1 ).

[0095] The underlying PDMS layer is a thin PDMS membrane (about 20 μm thick) containing micropores. The PDMS underlayer was made by spin-coating a thin layer of PDMS precursor (siloxane monomer: curing agent = 20:1) on the surface of the second silicon master. The silicon master contains an array of micropillars with a diameter of 200 μm. The micropillar size determines the micropore size of the ...

Embodiment 2

[0097] Example 2: Fabrication of polydopamine microspots on fluoropolymer membranes

[0098] The dopamine used in this example was purchased from Sigma-Aldrich Company, and the FEP (20 μm) used was purchased from Shanghai Yuyisong Plastic Products Co., Ltd.

[0099] Prepare fresh dopamine solution (pH 8.5, 2 g / L). A 1 cm x 1 cm FEP membrane was washed three times with ultrasonic waves passing through surfactant, ethanol and deionized water before use.

[0100] The PDMS microfluidic system prepared in Example 1 was tightly combined with the FEP membrane by the clamp method, and degassed in a vacuum desiccator for 30 minutes ( figure 2 a). After completing the degassing step, fresh dopamine solution (pH = 8.5) was introduced into the microfluidic system. The solution immediately fills the upper channel layer and then gradually fills the empty space in the lower micropores. The degassed PDMS system accelerated the filling process by adsorbing air in the microchannels and por...

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Abstract

A microarray substrate including a piece of fluoropolymer whose surface is modified with polydopamine, in which the polydopamine forms an array of microspots on the surface of the fluoropolymer piece, and allows immobilization of molecules or cells. A microarray including the substrate, a microfluidic system designed for dispensing reagents onto selected locations on the surface of substrates, and a method for preparing the substrate and the microarray, in which a dopamine solution is dispensed onto the fluoropolymer piece using the microfluidic system, and forms an array of polydopamine microspots serving as the reaction sites for microarray analysis.

Description

[0001] related application [0002] This application claims the benefit of priority to US Provisional Application 61 / 859,896, filed July 30, 2013, the contents of which are hereby incorporated by reference in their entirety. technical field [0003] The present application relates to microarray substrates, microfluidic systems and microarrays comprising such substrates for detecting, analyzing or studying molecules or particles of interest, especially biological materials. The present application also relates to methods for preparing substrates and microarrays and methods for detecting or analyzing molecules of interest in a sample using microarrays comprising said substrates. Background technique [0004] Known in the art, a series of fluoropolymers including polytetrafluoroethylene (PTFE), fluorinated ethylene propylene (FEP), perfluoroalkoxy (perfluoroalkoxy, PFA), etc. have excellent non-stick properties and can be used as Non-stick coating. PTFE is commonly used in no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/54393C40B20/02C40B40/02C40B40/04C40B40/06C40B40/08C40B40/10G01N33/54353G01N33/545
Inventor 郑波冯汇
Owner THE CHINESE UNIVERSITY OF HONG KONG