Lanthanide metal europium ion complex probe-based method for detecting pH value

A technology of lanthanide metal and detection method, which is applied in the field of chemical analysis to achieve the effects of rapid pH value response, low price and guaranteed repeatability

Inactive Publication Date: 2015-02-18
SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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  • Abstract
  • Description
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Problems solved by technology

[0005] In order to effectively reduce the problems of the detection signal being susceptible to interference, instability, and strong background signal in the detection of the pH value in the environmental water body, and to improve the recognizability and stability of the detection signal, the purpose of the present invention is to provide a pH value detection method based on lanthanide metal europium ion complex probe

Method used

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  • Lanthanide metal europium ion complex probe-based method for detecting pH value
  • Lanthanide metal europium ion complex probe-based method for detecting pH value
  • Lanthanide metal europium ion complex probe-based method for detecting pH value

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Eu(TTA) 3 (5-NH 2 -phen) preparation, concrete steps are as follows:

[0046] a. 5-NH with 1,10-phenanthroline (phen) as raw material 2 Synthesis of -phen

[0047] Synthetic steps: (1) Nitrolation of phen. Add 13mL of concentrated sulfuric acid to a 250mL three-necked flask, add 2.0g of phen in batches under magnetic stirring conditions, dissolve with magnetic stirring, and slowly raise the temperature. When the temperature rises to 150°C, slowly add 5.5mL of concentrated nitric acid. ℃, continue to react for 45min. After the reaction is complete, carefully pour the reaction solution into 130 g of ice-water mixture, and after the crushed ice is completely melted, use 2.0 mol·L -1 Adjust the pH value of NaOH solution to 7.0. At this time, a large amount of yellow precipitates are formed, which are filtered under reduced pressure, washed with water, and air-dried to obtain crude NO 2 -phen, recrystallized twice with ethanol to obtain yellow crystals, dried in vacuum...

Embodiment 2

[0050] Example 2: Stability study of ETN time-resolved fluorescence intensity

[0051] Take 10 different 10mL colorimetric tubes, add 1.0mL ETN stock solution respectively, and put them in a 60°C oven to remove the ethanol solvent. Then use B–R buffer with a pH value of 7.5 to adjust the volume to the mark, fully dissolve and mix evenly, and let it stand at room temperature for 10 minutes. After the ETN is fully dissolved and stabilized, take 3.0mL of the solution and add it to a 1-cm cuvette. , using a Perkin-Elmer LS-55 fluorescent phosphorescence spectrophotometer to measure and record the time-resolved fluorescence intensity changes. The maximum excitation wavelength and maximum emission wavelength are 355nm and 611nm, respectively, the slit is 5.0nm, and the delay time is 0.1ms. Thereby, 10 different time-resolved fluorescence intensity values ​​were obtained, and the relative standard deviation was calculated, and the relative standard deviation was 3.2%, which was less ...

Embodiment 3

[0052] Example 3: Research on the Effect of Dissolved Oxygen and Ionic Strength

[0053] a. Effect of dissolved oxygen on the time-resolved fluorescence intensity of ETN

[0054] In this example, three different dissolved oxygen solutions were prepared, namely oxygen saturated solution, nitrogen saturated solution and air saturated solution. The pH values ​​of the three solutions were controlled at 7.0. Then, the time-resolved fluorescence intensity of ETN in these three solutions was measured according to the steps and methods in Example 2, so as to study its influence. The results are recorded in the attached image 3 in Figure A. The results show that the time-resolved fluorescence intensities of ETN in the three solutions are basically unchanged, thereby fully illustrating that the interference of the oxygen concentration in the solution to the detection method can be ignored, and the influence of dissolved oxygen on the present invention can be ignored in actual detect...

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Abstract

The invention discloses a lanthanide metal europium ion complex probe-based method for detecting a pH value. The detection method mainly comprises the following steps: firstly, carrying out fluorescence intensity scanning on a to-be-detected sample A including lanthanide metal europium ion complexes and solutions with different pH values; observing the change of the fluorescence intensity, and carrying out quantitative detection on the pH values of the solutions, so as to obtain a linear interval between the pH values and the fluorescence intensity; and detecting the fluorescence intensity of a to-be-detected sample B including the lanthanide metal europium ion complexes and a to-be-detected water body sample, so as to obtain the pH value of the to-be-detected water body sample. The method disclosed by the invention is capable of achieving the detection effect of a traditional method, and has good selectivity, high response speed, short detection time, simplicity of operation and low price; a time-resolved fluorescence signal is strong and stable; and the repeatability of an experimental method and the recognizable degree of the fluorescence signal in a complicated environment are ensured, so that the ultimate practicability of the detection method is powerfully guaranteed.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis, and in particular relates to a pH value detection method based on a lanthanide metal europium ion complex probe. Background technique [0002] The pH value, or acidity and alkalinity, is an important indicator of water quality. The pH value of natural water is mostly between 6 and 9; the pH value of drinking water is required to be between 6.5 and 8.5; the pH value of some industrial water should be guaranteed between 7.0 and 8.5, otherwise it will have a corrosive effect on metal equipment and pipelines . There are both differences and connections between pH and acidity and alkalinity. The pH value indicates how acidic or alkaline the water is, while the acidity and alkalinity are the amount of acid or alkali substances contained in the water. Changes in pH in water quality indicate the degree of water pollution. In aquaculture water, the pH value directly reflects changes in water...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 黄大伟易皓邴永鑫洪伟虢清伟崔恺张政科卓琼芳牛承岗
Owner SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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