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Preparation method of disease-resistant production-increasing complex microbial inoculant

A technology of compound microbial bacterial agent and powder, which is applied in the field of preparation of compound microbial bacterial agent, can solve the problems of single function of bacterial agent and short shelf life, etc., achieve broad-spectrum effect, increase yield, and suppress disease

Inactive Publication Date: 2015-02-25
HEILONGJIANG HEIKE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem of single function and short shelf life of the existing bacterial agents, and provides a preparation method of a disease-resistant and production-increasing compound microbial bacterial agent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0018] Specific embodiment one: the preparation method of the disease-resistant and production-increasing compound microbial bacterial agent of this embodiment is carried out according to the following steps:

[0019] One, the fly ash and humic acid are mixed and sterilized evenly in a weight ratio of 4:6 to obtain an adsorption carrier;

[0020] 2. Use an inoculation loop to pick Pseudomonas fluorescens and inoculate it into 5mL King’s B medium, culture it at 30°C and 150r / min for 18 hours, and then streak and inoculate Pseudomonas fluorescens on the King’s B medium plate above, cultured in a 30°C incubator for 18 hours, picked a single colony and inoculated it into 5mL King’s B medium again, and cultured it at 30°C with 150r / min shaking for 18 hours to obtain a culture solution of Pseudomonas fluorescens; The Pseudomonas culture solution was inoculated into 100mL King's B medium according to the inoculation amount of 5% by volume, and cultured with shaking at 30°C and 150r / m...

specific Embodiment approach 2

[0027] Specific embodiment two: the difference between this embodiment and specific embodiment one is: King's B medium is made up of 20g peptone, 1.5g dipotassium hydrogen phosphate, 1.5g magnesium sulfate, 10g agar and 1000mL sterile water in the step 2, The pH was 7.2 ± 0.2. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0028] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: in step 2, the culture temperature in the fermenter is 28.5°C. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a preparation method of a disease-resistant production-increasing complex microbial inoculant and relates to a preparation method of a complex microbial inoculant. The problem that the existing bactericide is unique in function and short in shelf life is solved. The method comprises the following steps: step one, mixing coal ash and humic acid to realize sterilization to obtain an adsorption carrier; step two, inoculating pseudomonas fluorescens into a King's B culture medium for culture, preparing a pseudomonas fluorescens culture solution; preparing a pseudomonas fluorescens seed solution; fermenting in a fermentation tank to obtain pseudomonas fluorescens fermentation solution; mixing the pseudomonas fluorescens fermentation solution with the adsorption carrier to obtain powder containing pseudomonas fluorescens; step three, inoculating colloid bacillus into the culture medium for culture to prepare colloid bacillus culture solution; preparing colloid bacillus seed solution; culturing in a fermentation tank to obtain colloid bacillus fermentation solution; mixing the colloid bacillus fermentation solution with the adsorption carrier to obtain the powder containing the colloid bacillus; step four, compounding strains to prepare the complex microbial inoculant powder. The complex microbial inoculant is used for disease resistance and production increase of the crops.

Description

technical field [0001] The invention relates to a preparation method of a composite microbial bacterial agent. Background technique [0002] Chemical fertilizer is one of the main factors to increase grain production. Excessive and unreasonable application over the years has led to soil compaction, soil fertility decline, serious disease and quality decline. On the other hand, with the continuous development of science and technology and economy, people's requirements for living standards are also constantly improving, and the demand for diversified, high-quality, and harmless agricultural products has been put forward. Green and safe food has become a hot spot and fashion of consumption. . To improve the environment and develop ecological agriculture, there is an urgent need to improve planting technology, completely change the previous practice of relying on a large number of chemical fertilizers and pesticides to increase crop yields and produce high-volume and low-quali...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20A01N63/00A01P3/00C05F17/00C12R1/39C12R1/07
CPCA01N63/00C05F11/08C12N1/205C12R2001/07C12R2001/39Y02W30/40
Inventor 王东凯吴琼赵金海王雷曹涤非黄国庆李瑶孙尧薛佳莹
Owner HEILONGJIANG HEIKE TECH CO LTD