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noey2 gene mutation detection system and its kit

A systematic and mutant technology, applied in the biological field, can solve problems such as deletion and down-regulation of ARHI gene expression

Active Publication Date: 2016-08-24
山东国九堂制药集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have also shown that ARHI gene expression is often down-regulated or lost in ovarian cancer tissues; the loss of ARCH1 expression may also be related to the metastasis mechanism of breast cancer; ARHI also has a high proportion of loss in pancreatic cancer and other tissues

Method used

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  • noey2 gene mutation detection system and its kit
  • noey2 gene mutation detection system and its kit

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Embodiment

[0067] 1. The composition of the kit.

[0068] The NOEY2 gene mutation detection kit of this embodiment includes a PCR reaction solution, a primer mixture, a mutant control solution (positive control solution) and a wild-type control solution (negative control solution). The PCR reaction solution, primer mixture, mutant control solution and wild-type control solution were placed in different test tubes, as shown in Table 1.

[0069] Table 1 Kit composition table

[0070]

[0071] The description of each component of the kit in the above Table 1 is as follows:

[0072] 1. The PCR reaction solution includes 2×HRM Master Mix (manufactured by Roche, Cat#04 909 631 001) and 25mM MgCl 2 .

[0073] 2. The primer mixture includes forward detection primer NOEY1F and reverse detection primer NOEY1R for detecting NOEY2 5205T>C mutation site, forward detection primer NOEY2F and reverse detection primer for detecting NOEY2 5813 DEL A mutation site NOEY2R, forward detection primer NO...

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Abstract

The invention relates to a NOEY2 gene mutation detection system and a kit thereof. The NOEY2 gene mutation detection system comprises primer sequences used for detecting four mutation sites including NOEY25205T>C, 5813DELA, 6141G>A and 5270G>A; and the NOEY2 gene mutation detection system further comprises corresponding wild-type and mutant contrasts which are high in reliability, easy and convenient to obtain and capable of being applied in a large-scale manner. By means of the NOEY2 gene mutation detection system and the kit of the NOEY2 gene mutation detection system, an HRM reaction system used for detection is disclosed; detection of the multiple mutation sites can be covered; the specificity is high; and the NOEY2 gene mutation detection system and the kit have the beneficial effects of being rapid, accurate, high in sensitivity and the like.

Description

technical field [0001] The invention relates to a gene mutation detection product and specific primers used in the product, and belongs to the field of biotechnology. Background technique [0002] ARHI (Aplysia Ras Homology member I) gene is a maternally imprinted tumor suppressor gene discovered in 1999, also known as NOEY2, located at 1p31, about 8kb, including a promoter, two exons and an intron, with A 687bp protein coding region encodes a 26KD small GTP-binding protein, which is a member of the ras / rap superfamily and has 50-60% family homology with members of this family. It is found in liver, pancreas, brain, bladder and prostate It is expressed in various human tissues, and the expression of ARHI is the highest in normal ovarian tissue. [0003] ARHI (NOEY2) is equivalent to a functional haploid. In normal cells, CpG island methylation modification occurs on the maternal ARHI allele, so that its transcriptional activity is inhibited and not expressed, but it can rel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/156
Inventor 王明
Owner 山东国九堂制药集团股份有限公司