Application of novel moderate temperature alpha-amylase

An amylase and a technology for processing starch, which are applied in the fields of starch and starch sugar product preparation technology, food additive technology and starch-containing product processing fields, can solve problems such as poor effect, and achieve the effects of prolonging shelf life, good application value and maintaining softness

Inactive Publication Date: 2015-03-11
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the product of fungal amylase is maltose, which is le

Method used

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  • Application of novel moderate temperature alpha-amylase
  • Application of novel moderate temperature alpha-amylase
  • Application of novel moderate temperature alpha-amylase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation of embodiment 1 novel α-amylase

[0023] For details, see Examples 1-2 in the description of Chinese Invention Patent 201310043628.5.

Embodiment 2

[0024] Embodiment 2. Novel α-amylase activity assay and product thin plate chromatography analysis

[0025] 1.0ml 0.5% soluble starch (50mM pH7.0 Tris-HCl buffer solution) (heated to 50°C first), add 1μl enzyme solution (after diluting the original solution 10 times), react at 50°C for 10min, add 2ml DNS to make up to After 3ml, boiled water bath for 5min, cooled, at OD 540 The absorbance value was measured under the following method, and the control group was heat-inactivated 1 μl enzyme solution (after the original solution was diluted 10 times), and the treatment was the same as above. The measured amylase activity was calculated according to the glucose standard curve, and the amount of enzyme required to produce 1 μmol of reducing sugar per minute at 50°C was defined as an activity unit.

[0026] Amylase AmyM handles starch 10 minutes, utilizes thin plate chromatography (TLC) to analyze its product component after 30 minutes and 1 hour, TLC condition is: developing agent...

Embodiment 3

[0027] Example 3. Novel α‐amylase hydrolyzes starch to produce maltooligosaccharides based on maltohexaose

[0028] Make waxy corn starch (not limited to waxy corn) into 8% suspension, adjust the pH value to 6 with phosphate buffer solution, place it in a super constant temperature water bath at 99°C until it is completely gelatinized, and then completely gelatinize After the starch paste was cooled to 50°C, α-amylase AmyM was added, and the amount of α-amylase added was 5% of the dry starch base (equivalent to 100U per g dry starch base), and the products were collected for 3 and 12 hours, respectively. After 12 hours of enzymatic hydrolysis, the temperature was raised to 100°C for 20 minutes to inactivate the enzyme, and the collected product was analyzed using a KS803 column. The chromatographic column is Shodex KS‐803 (8mm×300mm), and the detector is a differential detector. The sample was prepared into a 2g / L solution, passed through a 0.45μm membrane, with ultrapure wat...

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Abstract

The invention discloses application of novel moderate temperature alpha-amylase, that is the invention discloses application of alpha-amylase AmyM of which the amino acid sequence is shown in SEQ ID No.1, for preparing syrup which is rich in maltohexaose through processingh. The syrup comprises the following components: more than 50% of oligosaccharide(higher than maltotetrose, 8-10% of maltotriose, lower than 6% of malt sugar, and lower than 35% of other kinds of oligosaccharide. When the syrup prepared from the amylase disclosed by the invention is compared with the conventional maltohexaose syrup, the glucose content is lower, the maltohexaose content is higher, the sugar components after enzyme treatment are relative single, and the application range is wider. Due to the good characteristic of the novel alpha-amylase AmyM disclosed by the invention, so that the preparation process of the oligosaccharide is simple, and products are easy to purify.

Description

technical field [0001] The invention belongs to the field of preparation technology of starch and starch sugar products, food additive technology and processing of starch-containing products, and relates to the application of a novel medium-temperature α-amylase, in particular to the application of a novel medium-temperature α-amylase in the treatment of various starches to prepare rich Application of maltohexaose-containing syrup. Background technique [0002] Maltooligosaccharides generally refer to polymers composed of 3‐9 monosaccharides, including maltotriose, maltotetraose, maltopentaose, and maltohexaose. Maltoligosaccharide syrup is mainly made of high-quality corn starch, which is obtained by amylase hydrolysis. The product has low sweetness and good taste. The maltooligosaccharide prepared by enzymatic hydrolysis has a slow rise in blood sugar and low calorie after consumption, and has a good health care effect on diabetics, cardiovascular patients and obese peopl...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04C12P19/12C12P19/02C12C11/00A21D8/04
Inventor 崔中利李周坤黄彦
Owner NANJING AGRICULTURAL UNIVERSITY
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