Method for preparing influenza vaccine

An influenza vaccine and vaccine technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, pharmaceutical formulations, etc., can solve problems such as long production cycle and hidden safety hazards of vaccines, and achieve short production cycle, difficult processing, and production. Simple and stable process

Inactive Publication Date: 2015-03-25
CHENGDU VERO BIOTECH
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a method for preparing influenza vaccine, so as to overcome the problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing influenza vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] Such as figure 1 Shown, a kind of method for preparing influenza vaccine is characterized in that, comprises the following steps:

[0024] 1) Preparation of seed poison

[0025] a) Passage and culture of cells: Digest and passage Vero cells with Thermo scientific HyQTase-trypsin cell dispersion, and continue to culture with HycloneSFM4MegaVir or equivalent serum-free medium cell growth medium to form monolayer cells;

[0026] b) Inoculation and propagation of seed virus: use cell maintenance solution to dilute the H1, H3 and B-type epidemic strains provided by WHO in 10 -4 Inoculate the well-growing monolayer cells above, and add 3ug / L Type IX trypsin at the same time, continue to cultivate, and harvest the virus liquid when the cells are more than 50--100% diseased; then use the virus liquid as a seed virus on the cells Carry out cell adaptive continuous passage for 6 generations as production seeds;

[0027] 2) Microcarrier suspension culture of Vero cells in a bio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing an influenza vaccine. The method comprises the following steps: preparing virus seeds; conducting micro-carrier suspension cultivation on Vero cells in a bioreactor; breeding a vaccine-made virus solution; inactivating and purifying the virus solution. The method adopts a cell line to replace a chicken embryo tissue for cultivation to prepare avian influenza viruses, and the problem that a chicken embryo itself has high possibility of being polluted by exogenous viruses can be solved; by the strict control on raw materials and the cultivation condition, the purity of prepared vaccine is guaranteed, and safety of the vaccine is ensured; the method adopts the bioreactor for vaccine preparation, is high in automation degree and simple and stable in preparation technology, and requires few operators required; the method can greatly reduce the preparation cost, is prevented from being restricted by raw material supply, and is short in preparation cycle; by adopting the method to prepare the vaccine, environmental pollution is less and is easy to handle; the conventional chick embryo preparation method can produce a great number of waste embryos and other waste, is great in handling difficulty, and relates to the problems of biological safety and public hygiene.

Description

technical field [0001] The invention relates to the field of influenza virus vaccines, in particular to a method for preparing influenza vaccines. Background technique [0002] The traditional method for preparing influenza virus vaccines is to use chicken embryos, which have been used in China for more than 50 years to cultivate vaccines. Currently used influenza vaccines are mainly inactivated influenza trivalent split vaccines prepared from chicken embryos, including influenza A strains, H3N2 and influenza B strains, and pandemic influenza vaccines. The main source of sick vaccine strains is determined by the surface antigens of influenza virus epidemic strains recommended by WHO according to the annual change of global influenza viruses for the production of influenza vaccines in the next year. The epidemic wild strains recommended by WHO and chicken embryos Double infection of high-yielding strains produces reassortment or anti-genetic reassortment, and new vaccine str...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K39/145C12N7/00A61P31/16C12R1/93
Inventor 高鹏符晓阳左红代荣涛
Owner CHENGDU VERO BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap