Special culture medium for oudemansiella canarri (jungh.) hohnel and oudemansiella canarri (jungh.) hohnel cultivation method
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A cultivation method and a technology for a mother seed culture medium are applied in the cultivation field of rare edible fungi, and can solve the problems of limited popularization of cultivation techniques, difficulty in realizing artificial cultivation, and few pale brown Ode mushrooms, etc., and are convenient for large-scale use and promotion. , the economic value is considerable, the effect of short fruiting cycle
Inactive Publication Date: 2015-03-25
GUANGDONG INST OF MICROORGANISM +1
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Problems solved by technology
[0004] At present, there are few research reports on Oudemansiella canarii, mainly due to the difficulty of artificial cultivation, and the difficulty of large-scale collection in the wild due to factors such as region and season.
At present, only Wang Shouxian et al. (patent application No. 201210429491.2) have disclosed the cultivation method and special medium of Oddya halva in China about the domestication and cultivation of Oddya pheascus. The mass ratio of wheat bran and slaked lime is 50:30:18:2, which is mixed with water to make a fruiting medium with a water content of 65%-70% (mass percentage). The cultivation medium formula includes apple Branch sawdust is very limited, and the promotion of cultivation techniques will be limited
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Embodiment 1
[0029]A special culture medium for Oddya palae, including the separation medium of the mother species, in terms of mass volume percentage, the separation medium of the mother species includes 1-2% peptone, 1.8-2.2% glucose, 2% agar, and the rest is broad Leaf sawdust leaching solution, wherein, when preparing the broad-leaved sawdust leaching solution, the mass of the broad-leaved sawdust is 10-20% of the mass volume percentage of the culture medium for separating the parent species.
Embodiment 2
[0031] The preparation of the isolation mother kind medium of embodiment 1:
[0032] (1) Preparation of broad-leaved sawdust leaching solution: dry broad-leaved branches and smash them to obtain broad-leaved sawdust, weigh the broad-leaved sawdust according to the ratio that the broad-leaved sawdust accounts for 10-20% of the mass volume percentage of the separation mother species culture medium, Then add water to boil the sawdust, the amount of water added should be less than the volume of the prepared separation mother seed medium, about 10 minutes after boiling, then filter out the sawdust residue to obtain the broad-leaved sawdust leaching solution;
[0033] (2) According to the mass-volume ratio of 1-2% peptone, 1.8-2.2% glucose, and 2% agar, each component is added into the broad-leaved sawdust leaching solution, dissolved evenly and then constant volume, to obtain a culture medium for separating mother species.
Embodiment 3
[0035] A special culture medium for Ouderda palae, including the production mother seed medium, in terms of mass volume percentage, the production mother seed medium includes 18-22% potato, 1.8-2.2% glucose, 2% agar, 0.1-0.2 % Potassium dihydrogen phosphate, 0.04-0.06% magnesium sulfate, 1-1.5% peptone, vitamin B1 trace, and the rest is water.
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Abstract
The invention provides a special culture medium for oudemansiella canarri (jungh.) hohnel. The special culture medium for oudemansiella canarri (jungh.) hohnel comprises a cultivation material culture medium, wherein the cultivation material comprises the following raw materials in percentage by weight: 45-50% of cotton seed hull, 30-35% of saw dust, 18-20% of bran, 1-2% of calcium carbonate and 1-2% of calcium sulphate. In addition, the special culture medium also comprises a mother strain separation culture medium, a mother strain production culture medium and a primary strain culture medium. The invention also provides an artificial cultivation method of oudemansiella canarri (jungh.) hohnel by using the culture medium. The method comprises the following steps: carrying out aseptic inoculation of primary strain of oudemansiella canarri (jungh.) hohnel to a cultivation material culture medium, culturing and producing mushrooms. The invention firstly provides the special culture medium for oudemansiella canarri (jungh.) hohnel with wide raw material sources and remarkable effect and provides an artificial domestication cultivation method using the special culture medium; the biotransformation rate reaches 110-130%. According to the cultivation method, the production period of mushrooms is short, large-scale application and popularization are convenient, and the economic values are high.
Description
technical field [0001] The invention belongs to the field of cultivation of rare edible fungi, and in particular relates to a special culture medium and a cultivation method of Oddella hazel. Background technique [0002] Oudemansiella canarii (Jungh.) Hohnel belongs to the Fungal Kingdom, the Phylum Fungi, Basidiomycotina, Phytomycetes, Agaricaceae, White Mushrooms, Oudemansiella genus, its fruiting bodies are generally medium and large, and the cap About 3-10cm in diameter, the surface is brown to tan, sticky when wet; the flesh is white; the gills are white to dirty white, relatively thin, unequal in length, straight to elongate, the fold margins are rough and brown to dark, and the stipe is often Curved, dirty white, with dark brown cilia and longitudinal stripes on the surface, soft to hollow inside; spores are ovoid, broadly ovoid to nearly spherical, about 12-23μm*10.5-18μm in size; frilled cysts up to 80- 150μm, the width can reach 12-40μm. Hazel Oddella often grow...
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