Recombinant bacteria for expressing plectasin and application thereof

A technology of plectasin and antibacterial agents, applied to recombinant bacteria expressing plectasia and its application fields, can solve the problems of increasing the difficulty of curing diseases, patients and social and economic burdens, and achieve the effect of increasing expression
CN104480059AActive Publication Date: 2015-04-01GUANGDONG HINAPHARM PHARMA CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGDONG HINAPHARM PHARMA CO LTD
Publication Date
2015-04-01

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Abstract

The invention discloses recombinant bacteria for expressing plectasin and an application thereof. The classification name of the recombinant bacteria is lactococcus lactis NZ9700-pGMN3, the preservation serial number of the recombinant bacteria is CCTCC NO: M2014611, the recombinant bacteria can achieve upper-high expression of plectasin protein, the expression quantity of the recombinant bacteria far exceeds that of other anode recombinant bacteria, and the recombinant bacteria can be directly used for preparing anti-bacteria medicament, have no hidden safety risks and hardly have any side effects. The recombinant bacteria have the functions of inhibition of methicillin-resistant staphylococcus aureus, streptococcus agalactiae, streptococcus pyogenes, enterococcus faecalis and the like. Bacterial strains of the recombinant bacteria can achieve efficient and continuous expression under self-induction, the recombinant bacteria can express and secrete fusion protein containing plectasin in the fermentation process, the total yield of protein is more than 1.41g / L, plectasin accounts for more than 70%, and the recombinant bacteria have the potential of treating mammitis and infectious diseases of a genital tract.
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Description

technical field

[0001] The invention relates to a strain of new bacterial strain and its application in treating inflammation, in particular to a strain of recombinant bacteria expressing plectasin and its application. Background technique

[0002] Lactic acid bacteria (lactis acid bacteria, LAB) is a class of Gram-positive bacteria that can ferment carbohydrates and produce lactic acid in large quantities. It includes more than 10 genera such as Lactococcus, Lactobacillus, and Bifidobacteria. Long-term application has proved its non-pathogenicity. Lactic acid bacteria are common bacteria in the intestines of humans and most animals, and are widely distributed in the digestive system, respiratory system, secretory system, oral system and feces.

[0003] Defensin is a host defense peptide widely present in organisms, which constitutes the first line of defense against pathogenic microorganisms. Generally 2-6kDa, rich in positively charged amino acid residues, containing 6-8...

Claims

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