A kind of detection method of maleic hydrazide residue in tobacco and tobacco products
A technology of maleic hydrazide and tobacco products, which is applied in the field of tobacco quality and safety, can solve the problems of lack of accurate control of pH value, etc., and achieve the effects of eliminating matrix effect, high sensitivity, and saving manpower and material resources
Active Publication Date: 2016-01-20
SHANGHAI TOBACCO GRP CO LTD
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Problems solved by technology
[0005] In view of the above-mentioned shortcoming of prior art, the object of the present invention is to provide a kind of detection method of maleic hydrazide residual amount in tobacco and tobacco product, be used to solve the lack of accurate control of pH value in the prior art, easy to operate, efficient Problems with high, accurate and simple quantitative methods for the detection of maleic hydrazide residues in tobacco and tobacco products
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Embodiment 1
[0053] 1 Reagents and instruments
[0054] 1.1 Reagents
[0055] Tobacco sample (provided by Shanghai Tobacco Group Co., Ltd. Technology Center); target: maleic hydrazide (250mg, Toronto Research Chemicals, Canada); internal standard: maleic hydrazide-d2 (50mg, Toronto Research Chemicals, Canada) Hydrochloric acid, acetic acid (analytical grade, Merck, USA); acetonitrile (chromatographically pure, Merck, USA); pure water (analytical grade, Merck, USA)
[0056] 1.2 Instruments
[0057] 1200 high performance liquid chromatography (Agilent Technology Co., Ltd., USA); API4000 mass spectrometer (Agilent Technology Co., Ltd., USA); MARS 12-bit microwave extractor (CEM Corporation, USA)
[0058] 2 Determination method
[0059] 2.1 Preparation of standard solution
[0060] Weigh the standard product of maleic hydrazide, add 1% acetic acid-water solution to constant volume, and prepare the standard stock solution. Wherein, first weigh 10 mg of maleic hydrazide standard substance, ...
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Abstract
The invention belongs to the field of quality safety of tobacco and particularly relates to a method for detecting maleic hydrazide residue in tobacco and a tobacco product. According to the method for detecting maleic hydrazide residue in tobacco and the tobacco product provided by the invention, microwave-assisted extraction as well as high performance liquid chromatography-mass spectrometry (HPLC-MS / MS) are adopted to determine the maleic hydrazide residue in tobacco and the tobacco product, and the residue is quantified by virtue of an internal standard curve method. Compared with the prior art, the method for detecting maleic hydrazide residue in tobacco and the tobacco product is high in extraction efficiency, simple and convenient to operate and accurate to quantify, has relatively high sensitivity, accuracy and precision, can be used for effectively improving the detection efficiency of maleic hydrazide residue in tobacco and the tobacco product and is very worth popularizing and applying.
Description
technical field [0001] The invention belongs to the field of tobacco quality and safety, in particular to a method for detecting residual maleic hydrazide in tobacco and tobacco products. Background technique [0002] Tobacco is a smoking food, and its pesticide residue problem has become an important part of smoking safety issues. The pesticide residue index of tobacco and tobacco products is an important content in the quality control of tobacco products in various countries, and has become an important part of tobacco leaf evaluation and selection in the international market. It is an important factor in the purchase of tobacco and an important content of commodity inspection in the international trade of tobacco. The pesticide maleic hydrazide is an important indicator for the detection of tobacco pesticide residues. In 2008, the Agricultural Chemicals Committee (ACAC) of the International Tobacco Scientific Research Cooperation Center (CORESTA) proposed 136 kinds of mal...
Claims
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IPC IPC(8): G01N30/88
Inventor 王磊君戚大伟费婷吴达刘百战
Owner SHANGHAI TOBACCO GRP CO LTD