A method for purification and rejuvenation of edible fungus strains
A technology for purifying and rejuvenating edible fungi, which is applied in botany equipment and methods, applications, horticulture, etc., can solve the problems of inability to rejuvenate edible fungus species, species degradation, and extinction, and achieve a good effect of species retention , high accuracy and simple formula
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Embodiment 1
[0023] Example of the purification and rejuvenation of Flammulina velutipes: In April 2013, the production of Flammulina velutipes produced by a company in Qingdao experienced a large reduction in production. Solve the infection of Flammulina velutipes and decide to adopt the inventive method to purify and rejuvenate Flammulina velutipes bacterial classification, concrete operation is as follows:
[0024] (1) Preparation of culture material
[0025] No. 1 compost: the percentage of sawdust weight is 97%, the percentage of river mud weight is 3%, and the above mixture is adjusted to 58% by using 1% potassium permanganate aqueous solution;
[0026] No. 2 compost: the percentage of sawdust weight is 97%, the percentage of river mud weight is 3%, and the above mixture is adjusted to between 58% using 0.5% thiophanate aqueous solution;
[0027] (2) Culture material production
[0028] (1) Prepare 2 bottomless test tubes;
[0029] (2) Take by weighing No. 1 compost and No. 2 comp...
Embodiment 2
[0041] Domestication of shiitake mushroom strains: A strain R&D center of a company in Qingdao collected a basswood shiitake mushroom with excellent species, and obtained 5 test tubes of the original mother species through tissue separation. Simultaneously accompanied by bacterium, mold infection, and this moment good species of Basswood shiitake mushrooms have disappeared, did not obtain this strain bacterial classification, decided to adopt the method of the present invention to purify bacterial classification, concrete operation is as follows:
[0042] (1) Preparation of culture material
[0043] No. 1 compost: the percentage of sawdust weight is 95%, the percentage of river mud weight is 5%, and the above mixture is adjusted to 50% by using 1% potassium permanganate aqueous solution;
[0044] No. 2 compost: the percentage of sawdust weight is 95%, the percentage of river mud weight is 5%, and the above mixture is adjusted to between 50% using 0.5% thiophanate aqueous solut...
Embodiment 3
[0059] Purification and storage of Pleurotus eryngii: The quality of Pleurotus eryngii developed by a company in Qingdao is very good, but after several passages, it is found that the quality has declined seriously. Therefore, it is necessary to purify and store the mother species for production. The specific operation is as follows:
[0060] (1) Preparation of culture material
[0061] No. 1 compost: the percentage of sawdust weight is 96%, and the percentage of river mud weight is 45%, and the above mixture is adjusted to 55% by using 1% potassium permanganate aqueous solution;
[0062] No. 2 compost: the percentage of sawdust weight is 96%, the percentage of river mud weight is 4%, and the above mixture is adjusted to between 55% using 0.5% thiophanate aqueous solution;
[0063] (2) Culture material production
[0064] (1) Prepare 2 bottomless test tubes;
[0065] (2) Take by weighing No. 1 compost and No. 2 compost respectively 80g;
[0066] (3) Push the weighed raw mater...
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