The invention relates to a method for the purification and rejuvenation of an
edible fungus strain. According to the method, a first culture medium and a second culture medium are adopted, wherein the first culture medium is obtained by mixing 95 to 97 weight percent of
sawdust and 3 to 5 weight percent of river
silt and regulating the
moisture content of the mixture with a 1 percent
potassium permanganate aqueous solution, and the second culture medium is obtained by mixing 95 to 97 weight percent of
sawdust and 3 to 5 weight percent of river
silt and regulating the
moisture content with a 0.5 percent topsin
aqueous solution. The method comprises the following steps: pushing 80 grams of the first culture medium and 80 grams of the second culture medium into the centers of bottomless test tubes respectively, and plugging cotton plugs into the two ends of the bottomless test tubes for sterilization; selecting
fungus blocks sized as green beans in an aseptic technique manner, inoculating the
fungus blocks into the first culture medium, performing culturing for 4 to 6 days at 25 DEG C, selecting
mycelium at the uninoculated end to be inoculated to one end of the second culture medium when the
mycelium in the first culture medium reaches the other end, performing culturing for 4 to 6 days at 25 DEG C, selecting the
mycelium at the uninoculated end to be inoculated to a PDA culture medium when the mycelium penetrates through the second culture medium, and performing culturing for 4 days at 25 DEG C to obtain sterile mother strain. The method is short in cycle and high in accuracy, the sources are simple, the
virus, bacterium and
fungus pollution of the strain can be rapidly removed, and meanwhile, rejuvenation and strain purification functions are realized.