A kind of preparation method of cell-containing nanofiber scaffold

A technology of nanofibers and cells, applied in medical science, prostheses, etc., can solve the problems of difficult distribution of cells, slow growth of new tissues, and low cell planting density, so as to facilitate signal transmission, adhesion, good controllability

Inactive Publication Date: 2016-08-24
上海蓝衍生物科技有限公司
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  • Claims
  • Application Information

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Problems solved by technology

However, the tissue engineering nanofiber scaffold obtained by this method has the following disadvantages: 1) The cell planting density is small, which makes the growth rate of new tissue slow
2) Tissues generally contain a variety of cells, and the fixed-point distribution of different types of cells is difficult to achieve
4) It is not suitable for the construction of large-size scaffolds. The cells in the center of the scaffold are adversely affected due to the reduction of liquid permeability, resulting in growth retardation, lack of vascularization, and eventually the phenomenon of "sandwich"

Method used

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  • A kind of preparation method of cell-containing nanofiber scaffold

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Embodiment 1

[0019] A method for preparing a cell-containing nanofiber scaffold. The nanofiber scaffold is composed of degradable polymers and cells, and is prepared at one time by electrospray technology, including the following steps:

[0020] 1) Use an electronic scale to weigh 1.76 grams of PVA particles and slowly add them to 18.24 grams of deionized water under stirring conditions. After fully swelling, heat up to about 90°C to accelerate dissolution, and keep warm for 3 hours to obtain transparent 8.8 After the %PVA solution, add 0.15 grams of β-TCP powder to mix and grind for 2 hours with a vibrating ball mill;

[0021] 2) High-temperature disinfection of the above materials at 120°C;

[0022] 3) Human adipose stem cells of passage 4 were digested with 0.25% trypsin and collected in a 50mL centrifuge tube, centrifuged at 1000 r / min for 5 min, discarded the supernatant, and transferred the cell suspension to the sterilized above materials and stirred evenly to obtain Cell-containin...

Embodiment 2

[0028] This example is basically the same as Example 1, except that: Weigh 2 grams of PEO particles with an electronic balance, slowly add them to 8 grams of deionized water under stirring, and heat up to about 60°C to accelerate dissolution after fully swelling. And keep it warm for 3 hours, after making a transparent 20% PEO solution, add 0.15 g of β-TCP powder for mixing and grind for 2 hours with a vibrating ball mill.

Embodiment 3

[0030] This example is basically the same as Example 1, except that the stem cells used in step 3) are bone marrow mesenchymal stem cells.

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Abstract

The invention relates to a preparation method of a cell-containing nanofiber scaffold. This method can realize the integrated processing of degradable biomaterials and cells, and directly utilizes high-voltage electrosprayed nanofibrous scaffolds containing cells. The specific steps are: (1) Use deionized water to prepare a water-soluble degradable polymer into a homogeneous solution, then add β-TCP powder for mixing and grind with a vibrating ball mill; (2) Sterilize the above materials with high-temperature steam; (3) Add the cell suspension to the above-mentioned sterilized material, and stir evenly; (4) Use a syringe to extract the above-mentioned cell-containing material and place it in a micropump, turn on the high-voltage power supply and the micropump, set the corresponding process parameters for EFI, and finally A nanofibrous scaffold containing cells is obtained. The entire preparation method of the invention is simple and easy to implement, and has great practical significance for rapid repair of bone defects and the like.

Description

technical field [0001] The invention relates to a method for preparing a cell-containing nanofiber scaffold, which belongs to the field of tissue engineering nanofiber scaffold preparation and repair. Background technique [0002] The electrospinning method is a method that can quickly and easily prepare nanofiber scaffolds that has emerged in recent years. The scaffold materials prepared by this technology have high porosity and good channel connectivity, which can well build a microenvironment for cell growth. Therefore, nanofibrous scaffolds are widely used in the medical field. The current common method for constructing nanofibrous tissue engineering nanofiber scaffolds is: first prepare nanofiber scaffolds, and then plant cells on the surface of the scaffolds. However, the tissue engineering nanofiber scaffold obtained by this method has the following disadvantages: 1) The cell planting density is small, which makes the growth rate of new tissue slow. 2) Tissues gener...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/18A61L27/16A61L27/50
Inventor 胡庆夕陈海萍刘媛媛向科
Owner 上海蓝衍生物科技有限公司
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