Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing active proteins of wheat germ cells

A technology of wheat germ protein and cell activity, which is applied to the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems that hinder the use effect of wheat germ protein solution, and achieve high active protein content, safe use, and high activity high effect

Active Publication Date: 2015-04-08
BEIJING 7SHOW TIMES TECH
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the existing wheat germ protein solution contains some miscellaneous proteins, such as fibrin, some glycoproteins, etc., which hinder the use effect of the wheat germ protein solution.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing active proteins of wheat germ cells
  • Method for preparing active proteins of wheat germ cells
  • Method for preparing active proteins of wheat germ cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Soak the wheat grains in distilled water, cultivate them at 28°C, cultivate them at an air humidity of 80%, cultivate them for 3 days, and crush them to 100 mesh to obtain a crushed mixture;

[0055] centrifuging the pulverized mixture at a speed of 5000r / min to obtain the first supernatant;

[0056] centrifuging the first supernatant at a speed of 8000r / min to obtain a second supernatant containing germ cells;

[0057] enzymatically hydrolyze the second supernatant, the temperature of enzymolysis is 27°C, and the time is 1h;

[0058] Wherein, the enzymolysis solution used in the enzymolysis contains carbohydrase, protease, lipase, 3-phytase;

[0059] The addition of carbohydrase is 1.2% of the second supernatant weight; The addition of protease is 0.1% of the second supernatant weight; The addition of lipase is 0.5% of the second supernatant weight; 3-muscle The amount of alcohol hexaphosphatase added is 0.15% of the weight of the second supernatant;

[0060] Under ...

Embodiment 2

[0071] Soak the wheat grains in distilled water, cultivate them at 35°C, cultivate them at an air humidity of 85%, cultivate them for 2 days, and crush them to 80 mesh to obtain a crushed mixture;

[0072] centrifuging the pulverized mixture at a speed of 5000r / min to obtain the first supernatant;

[0073] centrifuging the first supernatant at a speed of 8000r / min to obtain a second supernatant containing germ cells;

[0074] enzymolyze the second supernatant, the temperature of enzymolysis is 25°C, and the time is 2h;

[0075] Wherein, the enzymolysis solution used in the enzymolysis contains carbohydrase, protease, lipase, 3-phytase;

[0076] The addition of carbohydrase is 0.8% of the second supernatant weight; The addition of protease is 0.05% of the second supernatant weight; The addition of lipase is 0.4% of the second supernatant weight; 3-muscle The amount of alcohol hexaphosphatase added is 0.05% of the weight of the second supernatant;

[0077] Under the condition...

Embodiment 3

[0088] Soaking wheat grains in distilled water, cultivating at 30°C, cultivating at an air humidity of 85%, cultivating for 60 hours, crushing to 100 mesh, and obtaining a crushed mixture;

[0089] centrifuging the pulverized mixture at a speed of 5000r / min to obtain a first supernatant;

[0090] centrifuging the first supernatant at a speed of 8000r / min to obtain a second supernatant containing germ cells;

[0091] Enzymolyze the second supernatant, the temperature of the enzymolysis is 26°C, and the time is 1.5h;

[0092] Wherein, the enzymolysis solution used in the enzymolysis contains carbohydrase, protease, lipase, 3-phytase;

[0093] The addition of carbohydrase is 1% of the second supernatant weight; The addition of protease is 0.05% of the second supernatant weight; The addition of lipase is 0.5% of the second supernatant weight; 3-muscle The amount of alcohol hexaphosphatase added is 0.1% of the weight of the second supernatant;

[0094] Under the condition of 4°C...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of active protein preparation, and particularly to a method for preparing active proteins of wheat germ cells, comprising the following steps: adopting 35 percent of ammonium sulfate solution to carry out preformed precipitate to wheat germ protein solution, then centrifuging, and obtaining supernate; adopting saturated ammonium sulfate solution to fully precipitate the supernate, centrifuging, and obtaining precipitate and the supernate; dissolving the precipitate into PBS solution, then dialyzing, removing ammonium sulfate, and obtaining the wheat germ cell activity proteins. According to the preparation method, the ammonium sulfate solution of a certain concentration is chosen firstly to precipitate impure proteins such as fibrous protein, a portion of glycoprotein and a portion of other protein precipitation in the wheat germ protein solution, which are removed through centrifuging; then the saturated ammonium sulfate solution is reused to precipitate the glycoprotein, glycoprotein peptide and a portion of growth factor in the supernate, and the precipitate obtained is the wheat germ cell activity proteins; finally, a dialytic method is adopted to remove the ammonium sulfate in the precipitate, and pure wheat germ cell activity proteins are obtained.

Description

technical field [0001] The invention relates to the field of active protein preparation, in particular to a method for preparing wheat germ cell active protein. Background technique [0002] Wheat germ contains flavonoids, such as kaempferol, myricetin, luteolin, catechins, bitter terpenes, 17 amino acids, and 25 elements such as potassium, manganese, phosphorus, calcium, and iron. It has remarkable convenient effects in anti-aging and anti-oxidation. [0003] In the past, the extraction of wheat germ protein mainly used the method of salt-dissolving alkali extraction and acid precipitation. However, this method requires a large amount of acid and alkali, and discharges a large amount of nutrients such as sugar and acid-base wastewater, which makes post-processing difficult and seriously pollutes the environment. Moreover, it is easy to denature the protein during the extraction process; the existing wheat germ protein extraction adopts the reverse micellar method, and the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30C12P21/06
Inventor 黄海军
Owner BEIJING 7SHOW TIMES TECH