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Growth promoting endophytic beauveria bassiana strain and application thereof

A technology of Beauveria bassiana and Beauveria bassiana, applied in the application, microorganism, microorganism and other directions, can solve the problems of not seeing endophytic Beauveria bassiana, promoting the growth of host plants, etc., to reduce pesticide residues, promote The effect of growth and protection of the ecological environment

Inactive Publication Date: 2015-04-08
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no report that Beauveria bassiana promotes the growth of host plants.

Method used

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  • Growth promoting endophytic beauveria bassiana strain and application thereof
  • Growth promoting endophytic beauveria bassiana strain and application thereof
  • Growth promoting endophytic beauveria bassiana strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the colonization situation of endophytic coccidioides Beauveria bassiana in corn body by seed soaking method, as figure 1 Shown:

[0023] Select a plate of each strain of Beauveria bassiana with good spore production, scrape the spores with an inoculation loop and add them to 50ml of sterile water, vortex shaker, shake and mix well, filter, count, and adjust the concentration of the spore suspension to 5×10 7 pcs / ml, select corn seeds with full grains, soak them in spore suspension for 8 hours, plant the soaked corn seeds, make a mark, and treat them with sterile water as a control. After 20 days, the inoculum was isolated not only from the root, but also from the stem, leaf, and petiole, indicating that the fungus can invade the plant through the seed coat of corn, and the fungus can be transferred from the root to the stem and leaf .

Embodiment 2

[0024] Embodiment 2: endophytic coccidioides Beauveria bassiana promotes corn germination experiment, such as figure 2 Shown:

[0025] Select a plate of each strain of Beauveria bassiana with good spore production, and prepare a concentration of 1×10 6 spore suspension per ml; soak corn seeds with full grains and uniform size in 75% ethanol for 10 minutes, rinse with sterile water, and immerse in the spore suspension of Beauveria bassiana endophytic coccidioides, soak for 12 hours at room temperature Take it out, and use the seeds soaked in sterile water as a control.

[0026] Blot the excess suspension with sterilized absorbent paper, place in a Petri dish covered with sterile water to moisten the filter paper, 20 grains per dish, 5 dishes for each treatment, 5 repetitions, place at 25°C Cultivate in a light incubator for 3 days, record the number of germination every 12 hours, and calculate the germination potential and germination rate. The results showed that the germi...

Embodiment 3

[0027] Embodiment 3: Beauveria bassiana endophytic coccidioides promotes the growth experiment of corn seedlings;

[0028] Randomly select corn seeds, after surface disinfection, use a concentration of 1 × 10 7 Beauveria bassiana spore suspension per ml was soaked for 12 hours and then planted in sterilized vermiculite. Under 25°C greenhouse conditions, poured appropriate amount of sterile water. After 30 days, the number of seedlings, plant height, biomass and root activity were investigated. A total of 100 plants were treated with 5 repetitions. Seeds soaked in sterile water were used as controls. The results showed that the emergence rate, biomass, plant height, root activity and total chlorophyll content of plant leaves of Beauveria bassiana had a certain growth-promoting effect on corn, as shown in Table 1. image 3 , 4 , 5 shown.

[0029] Table 1 Effect of endophytic Beauveria bassiana on biomass and plant height of maize seedlings

[0030]

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Abstract

The invention discloses a growth promoting endophytic beauveria bassiana strain and application thereof. The endophytic beauveria bassiana strain is Beauveria bassiana Bb4, is registered and preserved in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on April 28, 2014, and the preservation number is CGMCC No.9097. The strain is inoculated to maize, wheat and the like, can colonize in a host body and promote the growth of the host plant, and has certain toxicity to corn borer larvae. Application of the strain to production of microbial fertilizer preparations is conducive to reducing pesticide residues and protecting the ecological environment.

Description

technical field [0001] The invention relates to a growth-promoting plant endophytic Beauveria bassiana strain and its application. Background technique [0002] Endophytic fungi can exert influence on plants through their own metabolites or existence itself by means of signaling, thus playing an important role in the complex ecosystem of "endophyte-plant-animal-environment". Many studies have shown that hosts infected with endophytic fungi often have advantages such as rapid growth, resistance to adversity, disease resistance, and animal damage, and are more competitive for survival than uninfected plants; and because endophytic fungi are widely distributed and diverse, therefore In recent years, endophytic fungi have aroused widespread interest from microbiologists, ecologists, and plant protectionists, and have gradually become a research hotspot at home and abroad; at present, it has been found that endophytic fungi have at least the following beneficial effects on ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 陈晓琳韩国民曹媛媛倪敬田孙乐妮常艳杨恩东邓瑞宁
Owner ANHUI AGRICULTURAL UNIVERSITY
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