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Complex microbial inoculant rich in grease raw material for medium-temperature biogas fermentation system and preparation method and application thereof

A compound bacterial agent and biogas fermentation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of LCFA toxicity

Active Publication Date: 2015-04-08
CHENGDU ZHONGKE ENERGY & ENVIRONMENTAL PROTECTION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen from Table 1 that the IC of heptanoic acid, capric acid and oleic acid 50 Smaller values ​​indicate greater toxicity of the three LCFAs

Method used

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  • Complex microbial inoculant rich in grease raw material for medium-temperature biogas fermentation system and preparation method and application thereof
  • Complex microbial inoculant rich in grease raw material for medium-temperature biogas fermentation system and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 The preparation of medium-temperature interoperating long-chain fatty acid oxidizing bacteria bacterial liquid

[0046] (1) Preparation of Syntrophomonas wolfei subsp.wolfei single bacterial solution

[0047] According to the medium formula numbered DSM No.213 provided by the German Culture Collection Center, the volume percentage content is 80% N 2 +20%CO 2 Prepare the liquid medium of Syntrophomonas wolfei subsp.wolfei under the anaerobic conditions, adjust the pH value to 7.2, and distribute it in 25mL anaerobic tubes, 500mL anaerobic bottles and 20L anaerobic tanks under the above anaerobic conditions. It is advisable not to exceed 20% of the total volume of the anaerobic tube, anaerobic bottle or anaerobic tank. Cover the airtight cap and sterilize at 121°C for 20 minutes. The tank is filled with 80% N by volume 2 +20%CO 2 The gas is inflated to an anaerobic tube or anaerobic bottle or anaerobic tank with a gas pressure of 100kPa. The live bacteri...

Embodiment 2

[0066] Example 2 Preparation of medium-temperature interoperating long-chain fatty acid oxidizing bacteria bacterium liquid

[0067] According to the needs of production, the scale of cultivation can be expanded and four-level cultivation can be carried out. Set 1m 3 The anaerobic tank is used as four-level culture, and the medium formula, inoculation method, culture conditions, and mixed volume ratio of the mixed bacterial solution of the corresponding bacterial species are the same as in Example 1.

Embodiment 3

[0068] Embodiment 3 Preparation of methanogen bacterium liquid of methanogenic hydrogen or acetic acid nutrition

[0069] (1) Preparation of Methanospirillum hungatei single bacterial solution

[0070] According to the DSM No.119 medium formula provided by the German Culture Collection Center, the volume percentage content is 80% N 2 +20%CO 2 Prepare the liquid medium of Methanospirillum hungatei under the anaerobic conditions, adjust the pH value to 7.2, and distribute it in 25mL anaerobic tubes, 500mL anaerobic bottles and 20L anaerobic tanks under the above anaerobic conditions, and the filling volume should not exceed 20% of the total volume of the anaerobic tube, anaerobic bottle or anaerobic tank is appropriate. Cover the sealed cap and sterilize at 121°C for 20 minutes. After sterilization and cooling, fill the anaerobic tube, anaerobic bottle, and anaerobic tank. Into the volume percentage of 80% N 2 +20%CO 2 The gas is inflated to an anaerobic tube or anaerobic bo...

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Abstract

The invention provides a complex microbial inoculant for a grease-rich raw material medium-temperature biogas fermentation system and a preparation method and application thereof. The complex microbial inoculant is mainly used for strengthening degradation (beta-oxidation) of long-chain fatty acid and methane production of a degradation product (acetic acid+CO2+H2) of the long-chain fatty acid, improving gas production rate of a grease-rich raw material, shortening fermentation dwell time and meanwhile avoiding severed accumulation of fermentation liquid upper-layer grease.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and relates to a compound bacterial agent used in a medium-temperature biogas fermentation system rich in oil and fat raw materials and its preparation method and application. The compound bacterial agent is mainly used to strengthen the gas production of the medium-temperature biogas fermentation system rich in oil and fat raw materials , improve the gas production efficiency of oil-rich raw materials. Background technique [0002] Because biogas fermentation technology can not only process various organic wastes, but also obtain clean and renewable energy such as biomethane, biogas fermentation technology has been widely used to treat and utilize various organic wastes, including kitchen waste, slaughter waste Organic waste rich in oil, such as waste, oil processing waste, etc. [0003] Compared with raw materials such as carbohydrates and proteins, although the methane yield o...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P39/00C12P5/02C12R1/01
CPCC12N1/20C12P5/023C12P39/00Y02E50/30
Inventor 黄亚军李东文昊深王加雷
Owner CHENGDU ZHONGKE ENERGY & ENVIRONMENTAL PROTECTION CO LTD
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