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Intermittent preparation method of camellia meal protein antihypertensive peptide

A technology for hypotensive peptides and camellia oleifera meal, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of low blood pressure-lowering activity and low yield of peptides, and achieve environmental protection and low production costs Effect

Inactive Publication Date: 2015-04-08
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the problems of low polypeptide yield and low blood pressure-lowering activity in the existing preparation methods of blood pressure-lowering peptides, and provide a batch-type preparation method of camellia oleifera meal protein blood-pressure-lowering peptides with higher yield, The obtained camellia oleifera meal protein has higher blood pressure-lowering peptide activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This embodiment includes the following steps:

[0024] (1) Camellia oleifera meal protein with low denaturation degree is adopted, and its nitrogen solubility index (NSI) reaches 96.0%, and the tea oleifera meal protein solution of 4.00% (Wt / Wt) is prepared; the preparation method of the camellia oleifera meal protein with low denaturation degree: Using the cold pressing method to produce oil at 61°C, the obtained camellia oleifera meal is extracted by alkali-soluble acid precipitation method, and dried under vacuum (0.035MPa) at low temperature (38°C) to obtain the camellia oleifera meal protein with low denaturation degree;

[0025] (2) Use 0.5mol / L NaOH to adjust the pH value of the camellia oleifera protein solution to 8.0, and control the temperature to 40°C. According to the quality of the camellia oleifera protein, add 0.30% (Wt / Wt) of the protein content in the camellia oleifera meal Compound protease I (alkaline protease, flavor protease and trypsin, the mass r...

Embodiment 2

[0036] This embodiment includes the following steps:

[0037] (1) Camellia oleifera meal protein with low degree of denaturation is adopted, its nitrogen solubility index (NSI) reaches 96.5%, and the tea oleifera meal protein solution of 4.25% (Wt / Wt) is prepared; the preparation method of the camellia oleifera meal protein of described low degree of denaturation: Using the cold pressing method to produce oil at 62°C, the obtained camellia oleifera meal is extracted by alkali-soluble acid precipitation method, and dried under vacuum (0.032MPa) at low temperature (39°C) to obtain low denaturation degree of camellia oleifera meal protein;

[0038] (2) Use 1 mol / L of HCl (in the content of the invention, 0.3-0.7 mol / L of NaOH is used to adjust the pH value, please modify it by the inventor) to adjust the pH value of the camellia oleifera protein solution to 8.2, and control the temperature to 42 ℃, according to the quality of camellia oleifera meal protein, add compound protease ...

Embodiment 3

[0049] This embodiment includes the following steps:

[0050] (1) Camellia oleifera meal protein with low degree of denaturation is adopted, and its nitrogen solubility index (NSI) reaches 97.0%, and a 4.50% (Wt / Wt) camellia oleifera protein solution is prepared;

[0051] The preparation method of the camellia oleifera protein with low degree of denaturation: use the cold pressing method to make oil at 63°C, and extract the camellia oleifera protein from the obtained camellia oleifera by alkali-soluble acid precipitation method, and vacuum (0.030MPa) at low temperature (40°C). Drying to obtain camellia oleifera meal protein with low denaturation degree;

[0052] (2) Use 0.5mol / L NaOH to adjust the pH value of the protein solution to 8.4, control the temperature to 44°C, and add a compound protease equivalent to 0.40% (Wt / Wt) of the protein content in the camellia oleifera meal according to the quality of the protein in the camellia oleifera meal I (alkaline protease, flavor p...

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Abstract

The invention discloses an intermittent preparation method of camellia meal protein antihypertensive peptide. The method comprises the steps: (1) preparing camellia meal protein solutions with the mass concentration of 3.0-6.0 percent by using camellia meal protein with low denaturalization; (2) performing enzymolysis on mixed enzyme of alkaline protease, flavourzyme and trypsase; (3) performing enzymolysis on neutral protease and papain; (4) performing enzymolysis on acid protease to obtain crude enzyme fluid; (5) performing flash evaporation and inactivation; (6) standing and centrifuging; (7) performing vacuum condensation on supernate at low temperature; (8) performing equilibrium dialysis desalination processing on the supernate; (9) filtering by using an ultrafiltration membrane, gathering filtering liquid, freezing and drying to obtain the camellia meal protein antihypertensive peptide. The camellia meal protein antihypertensive peptide prepared by using the intermittent preparation method has ash content of less than 1.0 wt%, protein recovery rate of more than or equal to 93.0 wt% and ACE inhibition rate of more than 90.0 percent, and has an obvious antihypertensive effect on spontaneously hypertensive rats (SHR).

Description

technical field [0001] The present invention relates to a preparation method of oil-tea camellia meal protein blood pressure-lowering peptide, in particular to a batch-type preparation method of meal cake protein blood pressure-lowering peptide. Background technique [0002] Hypertension (systolic blood pressure greater than 140 mmHg and / or diastolic blood pressure greater than 90 mmHg) is a worldwide cardiovascular disease that is highly associated with myocardial infarction, stroke, atherosclerosis, coronary heart disease, and stroke It is extremely harmful to the health of the body. In the regulation of peripheral vascular blood pressure, ACE (EC 3.4.15.1) plays an important role, and more and more evidences show that it is the main risk factor induced by cardiovascular disease. ACE is a dipeptide carboxyl peptidase, which is not only related to the regulation of blood pressure, but also related to cardiovascular function. In the renin-angiotensin system, by cleaving a ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34A61P9/12
Inventor 龚吉军黄卫文王挥唐静卢锟
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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