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Method for cultivating salt-tolerant rice by using pollen-tube pathway transformation technology

A technology of pollen tube passage and rice, which is applied in the field of cultivating salt-tolerant rice and using pollen pipe passage transformation technology to cultivate salt-tolerant rice, can solve the problems that still take time, achieve no genotype restriction, obvious effect, and easy to realize large-scale The effect of large-scale genetic transformation

Inactive Publication Date: 2015-04-22
林栖凤 +3
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Problems solved by technology

It is also reported that Chalermpol Kirdmanee, the director of the Plant Cell Technology Laboratory of the National Genetic Engineering and Biotechnology Center of Thailand, discovered 4 Thai perfume rice strains that can tolerate 2% NaCl in the laboratory in 2001, but there has been no follow-up relevant report about the latest progress ( See Transcriptional regulations of the genes of starch metabolism and physiological changes in response to salt stress rice (Oryza sativa L.) seedlings), in 2005, the Institute of Plant Physiology and Ecology, Shanghai Academy of Biological Sciences, Chinese Academy of Sciences announced that the study of rice salt-tolerant functional genes was achieved in Shanghai major breakthrough
In cooperation with American experts, the Institute successfully cloned the quantitative trait gene related to rice salt tolerance, but it will take some time before the actual application

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  • Method for cultivating salt-tolerant rice by using pollen-tube pathway transformation technology
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  • Method for cultivating salt-tolerant rice by using pollen-tube pathway transformation technology

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Embodiment Construction

[0033] Below in conjunction with accompanying drawing and specific embodiment the present invention will be described in further detail:

[0034] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0035] 1. Cultivation of salt-tolerant rice

[0036] 1. Materials

[0037] Donor: halophyte Phragmites reed

[0038] Receptors: Rice 9311, Yanhui 559, 7hai 76, 7hai 74

[0039] 2. The specific operation steps are as follows:

[0040] (1) DNA extraction from reed leaves by CTAB method

[0041] Weigh 10-15g reed leaves, add liquid nitrogen and grind into powder. Add 2-ME (mercaptoethanol) / CTAB extraction buffer (2% 2-ME V / V, 2% CTABW / V) preheated to 65°C , 100mmol / LTris·Cl pH8.0, 20mmol / L EDTA pH8.0, 1.4mol / L NaCl), mix well, add 1% PVP (polyvinylpyrrolidone W / V), keep the temperature at 65°C for 60min, add an equal volume of chloroform: Octanol (24:1, V / V) was extracted, centrifuged at 4°C, 8500r / min fo...

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Abstract

The present invention discloses a method for cultivating salt-tolerant rice by using a pollen-tube pathway transformation technology. The method comprises: (1) extracting bulrush leaf DNA; (2) transforming the exogenous DNA into acceptor rice; (3) carrying out pot screening at a germination period; (4) carrying out artificial salt pond screening and big field breeding; and (5) planting the stable strain in the salt marsh field. According to the present invention, the pollen-tube pathway technology is utilized to transfer the total DNA of the halophyte into the ordinary rice, such that the transferring of the super-distant genetic material is achieved, the tissue culture process is eliminated, a variety of unpredictable adverse effects possibly produced during the plant tissue culture process are avoided, multiple genes can be simultaneously imported, and the molecular breeding of the salt-tolerant plants can be achieved.

Description

technical field [0001] The invention relates to a method for cultivating salt-tolerant rice, which belongs to the technical field of plant breeding, and more specifically relates to a method for cultivating salt-tolerant rice by using the pollen tube channel conversion technology. Background technique [0002] Soil salinization is a serious problem affecting agriculture and ecological environment. The total area of ​​cultivated land in my country is 1.95 billion mu, 20% of which have different degrees of salinization and secondary salinization, and the tidal flats total more than 140 million mu. In addition, there are 520 million mu of inland saline-alkali soil, including 150 million mu of saline-alkali land. People have tried to transform saline-alkali land through reasonable drainage and irrigation, fresh water washing, and application of chemical modifiers, but it is often difficult to popularize because of the huge cost and little effect. So I turned my attention to di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/89A01H1/06
Inventor 林栖凤何登骥李冠一何加太
Owner 林栖凤
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