A kind of identification method of d1 type rice cytoplasmic sterile line and its hybrid combination

Abstract

The invention relates to the field of rice identification, in particular to a D1 type rice cytoplasmic male sterile line and an identification method of hybrid combination of the D1 type rice cytoplasmic male sterile line. Any one or more of base sequences of SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3 are used for designing primers so that gene amplification identification can be performed. According to the identification method, on the basis of genetic variation of rice mitochondrial genomes, firstly, mitochondria of the D1 type rice cytoplasmic male sterile line are extracted and sequenced, then, genomics analysis is performed on the mitochondria and other rice varieties, and three high-specificity Dongye type sterile line D1 A mitochondria specific sequences are screened out; primers are designed according to the sequences, and then whether the D1 type rice cytoplasmic male sterile line and a specific mark of the hybrid combination of the D1 type rice cytoplasmic male sterile line exist or not can be identified through a molecular detection means. Through the identification method, the D1 type rice cytoplasmic male sterile line and the hybrid combination of the D1 type rice cytoplasmic male sterile line can be fast, accurately, efficiently and sensitively detected, and the identification method is easy and convenient to operate, stable and reliable.

Description

technical field [0001] The invention relates to the field of rice identification, in particular to an identification method for a D1 type rice cytoplasmic sterile line and a hybrid combination thereof. Background technique [0002] In the 1970s, my country successfully applied the three-line method to cultivate hybrid rice, and promoted the planting on a large scale in production, realizing the second leap in my country's rice yield per unit area. From 1976 to 2005, the accumulative planting area of ​​hybrid rice in my country was 5.25 billion mu, and the rice harvest increased by about 650 million tons. Therefore, the development of hybrid rice is an important way to increase food production and solve food security problems (Yuan Longping, 2008). The basis for the use of three-line hybrid rice is cytoplasmic male sterility. Since the discovery of the wild-type cytoplasmic male sterile line and its widespread application, from the 1970s to the mid-1980s, breeders have bred a...

AI Technical Summary

Problems solved by technology

[0007] The D1 rice cytoplasmic sterile line is a sporophytic sterile line derived from Dongxiang wild rice. It is completely different from other types of sporophytic sterile lines that have been popularized and applied. At present, no corresponding specific molecular markers have been developed. Able to quickly identify the type of infertility

Images