Micro-fluidic chip for detecting fluoride in water body and preparation method thereof

A microfluidic chip and detection chip technology, applied in chemical instruments and methods, laboratory containers, color/spectral property measurement, etc., can solve the problem of no substantial breakthrough in fluoride, and achieve good application prospects , the effect of reducing consumption, reducing processing and detection time

Inactive Publication Date: 2015-04-22
HANGZHOU TINKER BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microfluidic chip technology that uses a wafer-shaped chip, prepares microstructures and microchannels on its surface for analysis, relies on centrifugal force to drive microfluidic flow, and completes the analysis of several samples at the same time, is currently used in the field of rapid detection of fluoride. No substantial breakthrough yet

Method used

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  • Micro-fluidic chip for detecting fluoride in water body and preparation method thereof
  • Micro-fluidic chip for detecting fluoride in water body and preparation method thereof
  • Micro-fluidic chip for detecting fluoride in water body and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Design and draw the microstructure and microchannel graphics of the microfluidic chip with computer-aided design software. Microstructures and microchannels were prepared on polymethyl methacrylate (PMMA) substrates by numerical control CNC micromachining system. Finally, the substrates were cleaned with tap water and distilled water respectively, and residual fingerprints, oil stains and other stains on the surface of the substrates were wiped with ethanol. Place 5ml of the sample to be tested in the sample cell, align, bond, press and seal the substrate with micropatterns, blank substrate and double-layer adhesive film to make a centrifugal microfluidic detection chip for detecting fluoride . Fix the microfluidic chip on the integrated platform of centrifuge rotation and detection, add fluorine reagent, 1 mL of acetate and lanthanum nitrate sodium solution to the sample injection hole, mix at a speed of 300 rpm, and react for 10 minutes. Increase the rotation speed t...

Embodiment 2

[0032] Design and draw the microstructure and microchannel graphics of the microfluidic chip with computer-aided design software. Microstructures and microchannels were prepared on polycarbonate (PC) substrates by a numerically controlled laser micromachining system. Finally, the substrates were cleaned with tap water and distilled water, and residual fingerprints, oil stains and other stains on the surface of the substrates were wiped with ethanol. Place 5ml of the sample to be tested in the sample cell, align, bond, press and seal the substrate with micropatterns, blank substrate and double-layer adhesive film to make a centrifugal microfluidic detection chip for detecting fluoride . Fix the microfluidic chip on the integrated platform of centrifuge rotation and detection, add fluorine reagent, 1 mL of acetate and lanthanum nitrate sodium solution to the sample injection hole, mix at a speed of 300 rpm, and react for 10 minutes. Increase the rotation speed to 1000 rpm, move...

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Abstract

The invention relates to a micro-fluidic chip for detecting fluoride in a water body and a preparation method thereof. The surface of the centrifugal type micro-fluidic chip is provided with a microstructure and a microchannel; and under the driving of centrifugal force generated by rotation of a centrifugal machine, the processes of mixing, reaction and separation of a sample to be detected and a reagent bag are realized, and an ultraviolet-visible spectrophotometer is used for quantitatively or qualitatively detecting the content of the fluoride in the sample on the chip. The micro-fluidic chip and the method have the advantages that a plurality of samples can be treated and detected simultaneously, the used amount of a reagent and the samples is few, and integration, miniaturization and automation of fluoride detection are realized; the micro-fluidic chip has the characteristics of economy, fastness and portability; and a whole-new analysis technology is provided for detecting the fluoride in the water body. The micro-fluidic chip and the preparation method are mainly applied in the fields of environmental protection and water-quality detection and the like.

Description

technical field [0001] The invention relates to a microfluidic chip for detecting fluoride in water and a preparation method thereof. The microfluidic chip has microstructures and microchannels on its surface, and is driven by the centrifugal force generated by the rotation of a centrifuge to realize the detection of the sample to be tested and the reagent package. The mixing, reaction and separation process of the microfluidic chip and the method can simultaneously process and detect multiple samples, and the amount of reagents and samples is less , has realized the integration, miniaturization and automation of fluoride detection, has the characteristics of economy, speed and portability, and provides a new analysis technology for the detection of fluoride in water. Mainly used in environmental protection, water quality testing and other fields. Background technique [0002] Fluoride is the main and important pollutant in electrolytic aluminum industry. After fluoride is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31B01L3/00
Inventor 王晓东叶嘉明吕志荣周海梦
Owner HANGZHOU TINKER BIOTECHNOLOGY CO LTD
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