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Eggplant fusarium wilt-resistant gene segments or gene markers and application thereof

A technology of gene markers and gene fragments, applied in the field of plant biotechnology science, can solve problems affecting the growth and development of eggplant plants, economic losses, etc., and achieve the effects of improving breeding efficiency, shortening the breeding period, and shortening the excavation cycle

Inactive Publication Date: 2015-04-29
CHANGSHU ZHILIN FRUITS & VEGETABLES PROFESSIONAL COOP
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Problems solved by technology

Fusarium wilt has seriously affected the normal growth and development process of eggplant plants, bringing great economic losses to production

Method used

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  • Eggplant fusarium wilt-resistant gene segments or gene markers and application thereof
  • Eggplant fusarium wilt-resistant gene segments or gene markers and application thereof
  • Eggplant fusarium wilt-resistant gene segments or gene markers and application thereof

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Embodiment Construction

[0017] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can rapidly identify eggplant fusarium wilt resistance genes. The specific implementation process is as follows:

[0018] 1. Extraction of DNA: Take an appropriate amount of young eggplant leaves, dry them with silica gel, grind them into powder, and extract the total genomic DNA by CTAB method. The extracted DNA was detected by an ultraviolet spectrophotometer, and samples with an OD260 / OD280 value of 1.8-2.0 were selected and stored at -20°C for future use.

[0019] 2. Amplification and cloning of the homologous fragment of the disease resistance gene: according to the amino acid sequence GG(L / I / V / M / S)GKTT and G(L / N) of the conserved region of the known NBS-LRR type disease resistance gene ) PL(A / T / S)(L / V), designed a pair of degenerate primers: NBS15'-GKTT GGIGGIWSIGGIAARAC...

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Abstract

The invention mainly relates to eggplant fusarium wilt-resistant gene segments or gene markers and application thereof. Three eggplant fusarium wilt-resistant gene segments or gene markers are rapidly separated by mainly adopting an anti-disease gene homologous sequencing method; and through sequencing, the lengths of nucleotides of these gene segments or gene markers are respectively 483bp, 501bp and 495bp. According to the eggplant fusarium wilt-resistant gene segments or gene markers, the excavating period of an eggplant fusarium wilt-resistant gene is effectively shortened; rapid identification of a wilt gene is facilitated; corresponding co-separation functional markers (SNP, SCAR and the like) are developed through identified wilt gene; the gene segments or gene markers can also be applied to assistant selection of molecular markers of the fusarium wilt-resistant gene; the accuracy is high; creation of multi-resistance breeding materials can be carried out by combining with molecular markers for other anti-disease genes; the breeding period is shortened; the breeding efficiency is improved; and a foundation is laid for elaboration of molecular mechanism of the eggplant fusarium wilt-resistant gene.

Description

technical field [0001] The present invention utilizes the homologous sequence method of disease-resistant genes to clone eggplant resistance to fusarium wilt gene fragments or convert them into molecular markers, etc., and mainly relates to conventional hybrid breeding of eggplant, molecular marker-assisted breeding, positioning and molecular mapping of resistance to fusarium wilt genes , the cloning of the Fusarium wilt resistance gene and the analysis of the regulatory mechanism of the disease resistance gene belong to the field of plant biotechnology science. Background technique [0002] Fusarium wilt is one of the most important diseases in eggplant production. The leaves of the diseased plants of eggplant wilt gradually turn yellow and wither from bottom to top, and the symptoms are mostly manifested on the first and second layers of branches. Sometimes only half of the same leaf turns yellow, and the other half is healthy as usual. The diseased stem is cross-sectione...

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Application Information

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IPC IPC(8): C12N15/29C12N15/11C12Q1/68
Inventor 钱孝英钱春桃
Owner CHANGSHU ZHILIN FRUITS & VEGETABLES PROFESSIONAL COOP