Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of golden flower nanoparticle immune chromatography test strip capable of simultaneously detecting deoxynivalenol and fumonisins B1 in grains

A technology of deoxynivalenol and nanoparticles, applied in measuring devices, analytical materials, instruments, etc., to achieve broad market prospects, simple operation, and high sensitivity

Inactive Publication Date: 2015-04-29
NANCHANG UNIV
View PDF6 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, there is no use of golden flower nanoparticles as antibody markers for the simultaneous detection of DON and FB. 1 However, the use of golden flower as an antibody marker for the rapid detection of mycotoxins has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. Simultaneous fast detection of DON and FB 1 Preparation of trial strips.

[0038] 1. DON and FB 1 Preparation of monoclonal antibody-colloidal gold labels.

[0039] (1) Preparation of golden flower nanoparticles.

[0040] Put 75 μL of chloroauric acid solution with a concentration of 100 mM (1g of chloroauric acid in 25ml of deionized water) into a conical flask containing 30 mL of deionized water, stir vigorously and heat to boiling, add 900 μL of a mass fraction of 1 % sodium citrate aqueous solution and keep boiling until the color of the solution turns red, and heat for 10 min. Bring to room temperature for later use.

[0041] For a typical synthetic sea urchin-like nanoparticle, 25 μL of aqueous chloroauric acid (100 mM) was placed in 9.6 mL of deionized water with vigorous stirring, followed by the addition of 50 μL of gold seeds, 22 μL of 1% citric acid Sodium, and 1000 μL of 30 mM hydroquinone (33 mg added to 10 mL of deionized water), the solut...

Embodiment 2

[0050] Example 2. DON and FB in the sample 1 simultaneous detection.

[0051] DON and FB in rice, wheat and corn 1 simultaneous detection.

[0052] (1) Sample pretreatment and testing.

[0053] Weigh 1.0 g of pulverized samples to be tested (such as rice, wheat and corn) into a conical flask, add 100 mL of distilled aqueous solution, shake well for 3-5 min, filter with qualitative filter paper, and collect the filtrate; then take 0.5 mL of Add the filtrate to 1 mL of distilled water, mix well, and use it as the solution to be tested.

[0054] Take the above solution to be tested, add 2 drops of anionic surfactant, 0.5% Tween-20 in PBS (0.05M) solution, as the sample solution.

[0055] Take the above-mentioned sample liquid and drop it into the sample hole of the test strip, and the test result can be observed after 5 minutes.

[0056] (2) Result analysis.

[0057] When the test strip shows three red lines, it means: the content of DON is lower than 1000 μg / kg, and the c...

Embodiment 3

[0058] Example 3. (Application example).

[0059] 1. Specificity experiment.

[0060] According to the method described in Example 2, the mycotoxins such as 3-AC-DON, Nivalenol, T-2, HT-2, AFB, OTA, Citrinin, α-Zearalenol and β-Zearalenol were tested with this patent test strip There were three red lines in the test, and the result was that the test strip had no crossability with 3-AC-DON, Nivalenol, T-2, HT-2, AFB, OTA, Citrinin, α-Zearalenol and β-Zearalenol and other mycotoxins reaction.

[0061] 2. Sensitivity experiment.

[0062] Using the method described in Implementation 2, use test strips to detect DON and FB at concentrations of 0, 0; 2, 2; 5, 5; 10, 10; and 20, 20 ng / mL, respectively 1 Mixed standards, repeated 10 times, wherein when the DON concentration is equal to or higher than 5 ng / mL, the DON detection line does not show red; and when the FB 1 At concentrations equal to or higher than 5 ng / mL, FB 1 The detection line does not show red. Therefore, the DO...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a golden flower nanoparticle immune chromatography test strip capable of simultaneously detecting deoxynivalenol and fumonisins B1 in grains. The method comprises the following steps: (1) preparing a DON monoclonal antibody-golden flower nanoparticle marker and a FB1-monoclonal antibody-golden flower nanoparticle marker; (2) treating a gold-marked conjugate release pad; (3) enveloping a nitrocellulose membrane; and (4) preparing the test strip. The immune chromatography test strip is high in specificity and sensitivity, visual and intuitive in detection result, simple, convenient and fast to operate, and easy to popularize and apply within a large range, and is widely suitable for the requirements such as grain purchase site inspection, food safety inspection and customs quarantine control.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the preparation of a rapid detection test strip for mycotoxins. Background technique [0002] Deoxynivalenol (DON) is a toxic secondary metabolite produced by Fusarium - one of the trichothecenes, belonging to the B-type trichothecenes. Fumonisin B 1 (Fumonisin FB 1 ) is another mycotoxin, a water-soluble metabolite produced by Fusarium moniliforme. The above two mycotoxins widely exist in grains and their products, and have teratogenic, carcinogenic, immunosuppressive toxicity, neurotoxicity and other effects. Health poses a potential threat. [0003] Currently about DON and FB 1 The detection methods mainly include: thin layer chromatography, gas chromatography, gas chromatography-mass spectrometry and liquid chromatography, liquid chromatography-mass spectrometry or liquid chromatography-mass spectrometry-mass spectrometry, etc.; and related FB 1 The detection methods mainly i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/577G01N33/531
CPCG01N33/54346G01N33/531G01N33/577
Inventor 黄志兵许杨李燕萍任文洁季艳伟何庆华涂追
Owner NANCHANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com