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Anti-bifenthrin monoclonal antibody and use thereof

A monoclonal antibody, bifenthrin technology, applied in the field of biology, can solve the problems of difficult to meet the rapid and simple detection of a large number of samples, cumbersome sample pretreatment, expensive detection instruments, etc., and achieves high accuracy, high sensitivity and specificity. strong effect

Inactive Publication Date: 2017-02-01
无锡市白蚁防治中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These instrumental analysis methods are sensitive and accurate, but the detection instruments are expensive, the sample pretreatment is cumbersome, the detection is time-consuming and laborious, and it is difficult to meet the needs of rapid and simple detection of a large number of samples.

Method used

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  • Anti-bifenthrin monoclonal antibody and use thereof
  • Anti-bifenthrin monoclonal antibody and use thereof
  • Anti-bifenthrin monoclonal antibody and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Preparation of anti-bifenthrin monoclonal antibody

[0020] 1. Mouse immunity:

[0021] (1) The immune hapten represented by formula (I) is coupled to BSA (bovine serum albumin) to prepare an immunogen;

[0022]

[0023] (2) First immunization: Take healthy BALB / c female mice aged 6-8 weeks, and mix the prepared immunogen with 1 mg / mL in PBS buffer, then mix with an equal volume of Freund’s complete adjuvant (FCA). After emulsification, each mouse was injected with 200μL (ie 100μg immunogen) through the abdominal cavity;

[0024] (3) Boost immunization: Three weeks after the initial immunization, the immunogen (the same dose as the first immunization) and an equal volume of Freund's incomplete adjuvant (FIA) are mixed, and after full emulsification, each mouse is injected with 200μL through the abdominal cavity Afterwards, every two weeks, boost immunization for 4 times; 3 days before cell fusion, mice were injected with immunogen, and the injection dose was the sam...

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Abstract

The invention relates to an anti-bifenthrin monoclonal antibody and an application thereof and especially provides a monoclonal antibody capable of specifically recognizing bifenthrin, a hybrid tumor generating the monoclonal antibody and the application of the monoclonal antibody. The invention belongs to the field of biology. A method includes following steps: immunizing BALB / c female mice by an artificial antigen; performing cell fusion with myeloma cell SP2 / 0 and mice spleen cells to prepare a hybridoma; obtaining a hybridoma strain which can stably secret the anti-bifenthrin monoclonal antibody; injecting the hybridoma strain into abdomen of the mice to prepare ascites; purifying the ascites in an octanoic acid-ammonium sulfate manner to obtain the specific anti-bifenthrin specifically monoclonal antibody. The monoclonal antibody does not cross-react with other compounds. ELISA method established by the antibody can be used for quickly, sensitively, conveniently and cheaply detecting residual bifenthrin in environment and agricultural products.

Description

Technical field [0001] The present invention relates to an anti-bifenthrin monoclonal antibody, especially a monoclonal antibody that can specifically recognize bifenthrin, a hybridoma that produces the monoclonal antibody, and the use of the monoclonal antibody, belonging to Biological technology field. Background technique [0002] Pyrethroids (Pyrethroids) insecticides are a kind of super-efficient insecticides imitated based on the chemical structure of natural pyrethroids. Due to their wide insecticidal spectrum, good effect, low residue, no accumulation, etc. Over the past 30 years, applications have become increasingly common. Pyrethroid insecticides are mainly used to kill pests on crops such as cotton, vegetables, fruit trees, tea, etc. It is a broad-spectrum and highly effective insecticide. The chemical name of bifenthrin is (1R,S)-cis-(Z)-3-(2-chloro-3,3,3-trifluoro-1-propenyl)-2,2-dimethyl -Cyclopropane carboxylic acid-2-methyl-3-phenylbenzyl ester, Bifenthrin, as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/44C12N5/20G01N33/577
Inventor 陆艺文王鸣华夏亚中华修德周秋君李伟
Owner 无锡市白蚁防治中心
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