Method for determining breast cancer

A technology of breast cancer and markers, applied in the field of breast cancer markers, can solve the problems of breast cancer markers that do not determine the accuracy of breast cancer diagnosis

Inactive Publication Date: 2015-04-29
WAKO PURE CHEMICAL INDUSTRIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a breast cancer marker, breast cancer markers with high diagnostic accuracy for breast cancer have not yet been identified

Method used

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  • Method for determining breast cancer
  • Method for determining breast cancer
  • Method for determining breast cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Screening of markers of embodiment 1 breast cancer

[0068] 1) Pretreatment of samples

[0069] Add each teat secretion (several μL) sampled from breast cancer patients (11 patients) to 100 μL of sample preservation solution (aqueous solution containing 50 mM ammonium bicarbonate and 0.02% sodium azide), and centrifuge at 18,000×g at 4°C. 5 minutes. After fractionating the supernatant, the protein concentration was determined by the ultraviolet absorption method using ultraviolet light having a wavelength of 280 nm, and then a solution having a protein concentration of 0.5 mg / mL was prepared with 50 mM ammonium bicarbonate aqueous solution. After adding 1.41 μL of a 45 mM dithiothreitol aqueous solution for reduction, trifluoroethanol was added to 50% to denature the protein, followed by heating at 60° C. for 1 hour. After returning to room temperature after heating, 1.41 μL of a 100 mM iodoacetamide aqueous solution was added, and alkylation was carried out at room t...

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Abstract

The present invention relates to: a breast cancer marker which is selected from the group consisting of carboxypeptidase N subunit 2, extracellular matrix protein 1, serum amyloid P component, nebulin, complement component C8 alpha chain, apolipoprotein L1, flavin reductase, catalase, carbonic anhydrase 2, apolipoprotein C-I, nuclear pore glycoprotein 210, superoxide dismutase [Cu-Zn], bisphosphoglycerate mutase, carbonic anhydrase 1 and peroxiredoxin 2; a method for determining breast cancer, which comprises detecting the breast cancer marker in a sample and determining breast cancer on the basis of the results of the detection; and a kit for use in the method.

Description

technical field [0001] The present invention relates to a novel breast cancer marker, a method for detecting the marker and identifying (diagnosing, screening) breast cancer according to the result, and a kit for the identification. Background technique [0002] At present, as a method of diagnosing breast cancer, there are diagnosis by detection of breast cancer markers in samples such as body fluids and blood, or diagnosis by mammography. [0003] For the method of diagnosing breast cancer by detecting breast cancer markers, carcinoembryonic antigen (CEA) is used as a marker, and the amount in a sample is measured with a diagnostic kit or the like, and breast cancer is diagnosed based on the result. However, this method lacks accuracy in showing breast cancer specificity, and has not yet become a practical definitive diagnosis of breast cancer. [0004] In addition, in Japanese Patent Laid-Open No. 2002-131322 (Patent Document 1), it is disclosed that mammary gland fluid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/53G01N33/574
CPCG01N33/57415C12Q1/28C12Q1/30C12Q1/527G01N2800/365G01N2800/52
Inventor 松浦修治松浦成昭黑野定金子有香
Owner WAKO PURE CHEMICAL INDUSTRIES
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