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Application of auxin on aspect of improvement of air formaldehyde pollution purification by potted plant

A plant purification and auxin technology, which is applied in the field of plant purification of air pollution, can solve the problems that affect the absorption and purification of formaldehyde by plants, low purification efficiency, cumbersome operation, etc., so as to improve the ability of plants to purify air formaldehyde pollution and increase the opening of stomata and conductance, the effect of simple operation

Inactive Publication Date: 2015-05-06
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are mainly methods for purifying formaldehyde such as adsorption, condensation, ozonation, photocatalytic degradation, catalytic combustion, and microbial purification. Plant purification indoor formaldehyde pollution is a cost-effective pollution remediation technology that meets the needs and psychology of the public
Plant purifying gas formaldehyde is mainly absorbed through stomata. The function of stomatal opening is determined by stomatal opening and conductance. Many biological and abiotic factors can inhibit stomatal opening and conductance, thus affecting the absorption and purification of formaldehyde by plants. function of

Method used

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  • Application of auxin on aspect of improvement of air formaldehyde pollution purification by potted plant
  • Application of auxin on aspect of improvement of air formaldehyde pollution purification by potted plant
  • Application of auxin on aspect of improvement of air formaldehyde pollution purification by potted plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the cultivation and processing of broad bean plant

[0021] 1. The experimental material is broad bean seedlings. After the broad bean seeds are germinated, they are sown in flowerpots mixed with red soil and perlite at a mass ratio of 6:1. Three germinated robust seeds are placed in each flowerpot, watered once every two days during the cultivation period, and water is applied after the leaves grow. One Hoagland's culture solution, used for this experiment when the seedlings grow to four pairs of leaves;

[0022] 2. Configure IAA treatment solutions with different concentrations (5, 10, 20, 40 μmol / L);

[0023] 3. Spray the lower epidermis of broad bean leaves with the above-mentioned concentration gradient treatment solution respectively. The lower epidermis is covered with water drops without dripping. After treating the IAA treatment solution for 8 hours, put the broad bean plants into the air containing 14.8 mg / m 3 In the glass airtight chamber...

Embodiment 2

[0025] Embodiment 2: adopt the broad bean leaf after the 3rd step processing in embodiment 1 to carry out PM H + -ATPase and H ＋ - Pump activity assay

[0026] 1. Plasma membrane protein extraction and concentration determination: The plasma membrane of broad bean leaves sprayed with different IAA concentrations was extracted using a kit from Beibo Reagent Co., Ltd. The plasma membrane protein concentration after extraction was measured by Bradford method, in 800 μL ddH 2 Add 5 μL of plasma membrane protein to O, mix well, then add 200 μL of Bradford solution, let it stand at room temperature for 10 minutes, detect the protein concentration at OD595 wavelength, and calculate the volume corresponding to 50 μg of plasma membrane protein.

[0027] 2. PM H + -ATPase activity assay steps are as follows:

[0028] (1) PM H + - The determination of ATPase activity was carried out in a 0.5 mL reaction system; the reaction system contained 50 mmol / L BTP / MES, 5 mmol / L MgSO 4 , 50 ...

Embodiment 3

[0035] Embodiment 3: Determination of leaf stomata opening and conductance

[0036] Get the blade that has been processed for 1 hour in the third step of Example 1, tear off the epidermis, place it on a cover glass, add a drop of physiological saline, cover the cover glass, observe and measure the stomatal opening under a medium-magnification microscope, 40 random measurements were taken for each concentration, and the average value ( image 3 A). The stomatal conductance was measured with a yaxin-1301 plant porometer (Beijing Yaxin Liyi Technology Co., Ltd.), and each treatment concentration was measured three times, and the average value ( image 3 B).

[0037] From image 3 A and image 3 In B, after spraying different concentrations of IAA, the stomatal opening and conductance of faba bean leaves were restored to varying degrees, but 20 μmol / L IAA had the largest recovery degree of stomatal opening and conductance of broad bean leaves.

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Abstract

The invention discloses an application of auxin IAA on the aspect of improvement of air formaldehyde pollution purification by a potted plant. When the application is used, IAA solution is sprayed on the lower epidermis of a potted broad bean leaf; in eight hours, a broad bean plant is respectively put in air environments with 14.8mg / m<3> of gas formaldehyde and 4mg / m<3> of gas formaldehyde; after the broad bean plant is processed for 1h in the stress environment of 14.8mg / m<3> of gas formaldehyde and is respectively processed for 24h,48h and 72h in the stress environment of 4mg / m<3> of gas formaldehyde, the physiological and biochemical indexes of the leaf are respectively measured; an experiment result shows that the plasmalemma ATP enzymatic activity, the H+- pump activities, the stomatal aperture and the conduction degree of the broad bean leaf growing in the formaldehyde pollution environment are all improved after the IAA is sprayed than a comparison plant, the changes are favorable for improving plant pore opening activities and improving an adsorption rate on the formaldehyde by the leaves so as to enhance a function of formaldehyde pollution purification by the plant.

Description

technical field [0001] The invention belongs to the field of plants purifying air pollution, and relates to a new application of auxin, in particular to a new application of auxin in improving formaldehyde pollution of air purification by plants. Background technique [0002] Indoor air pollution has become the third pollution period after "soot pollution" and "photochemical smog pollution". Among the many indoor organic pollutants, formaldehyde has become one of the ubiquitous and serious indoor pollutants due to its wide range of sources, great harm, and long duration. Relevant surveys show that the concentration of indoor formaldehyde in my country is generally higher than the indoor air quality standard. Indoor formaldehyde pollution has become the most important indoor air environmental problem in my country, seriously endangering people's lives and health. Purifying indoor air formaldehyde pollution has become a concern of people all the time. topic. At present, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06B01D53/84
CPCB01D53/84A01G7/06Y02A50/20
Inventor 陈丽梅孙慧群
Owner KUNMING UNIV OF SCI & TECH
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