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A method for improving sea cucumber culture cofferdam bottom quality

A cofferdam and bottom material technology, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of difficult sea cucumber breeding cofferdam bottom quality improvement, organic matter decomposition at the bottom of the breeding cofferdam is not obvious, etc.

Active Publication Date: 2018-10-09
DALIAN XINYULONG OCEAN TREASURES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The other is a reforming agent mainly based on oxygenates, which only temporarily relieves the anoxic state at the bottom of the aquaculture cofferdam, and has no obvious effect on the decomposition of organic matter at the bottom of the aquaculture cofferdam
Therefore, there are certain difficulties in applying these three types of substrate modification agents to the substrate modification of sea cucumber breeding cofferdams.

Method used

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  • A method for improving sea cucumber culture cofferdam bottom quality

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 Encapsulation of nitrosomonas

[0022] 1. Preparation of Nitrosomonas bacterial suspension

[0023] 1.1 Activation of Nitrosomonas strains

[0024] Nitrosomonas europaea WY21 was selected, and the preservation number was CGMCC No.9766. Pick a small amount of nitrosomonas strains preserved on the slant, and put them into a 100mL Erlenmeyer flask, and the volume of the seed culture medium is 20mL. The formula of the seed medium is ammonium sulfate 0.5g, sodium chloride 0.3g, potassium dihydrogen phosphate 1g, magnesium sulfate heptahydrate 0.03g ferrous sulfate heptahydrate 0.03g, calcium chloride 0.03g glucose 5g, water 1000mL, pH 7.5. The activation conditions are a temperature of 30° C., a rotation speed of 140 r / min, and a culture time of 16 hours.

[0025] 1.2 Fermentation of Nitrosomonas strains

[0026] Insert the activated seed culture solution into a 1L Erlenmeyer flask with an inoculum size of 4%, the liquid content of the fermentation medium i...

Embodiment 2

[0037] Embodiment two embedding bacillus

[0038] 1. Preparation of Bacillus suspension

[0039] 1.1 Activation of Bacillus strains

[0040]Pick a small amount of Bacillus strains preserved on the slant, put them into a 100mL Erlenmeyer flask, and fill the seed medium with a volume of 20mL. The formula of the seed medium is 1g of yeast extract, 0.5g of peptone, 0.01g of iron phosphate, 100mL of water, and pH 7.2. The activation conditions are a temperature of 30° C., a rotation speed of 160 r / min, and a culture time of 16 hours.

[0041] 1.2 Strain fermentation

[0042] Insert the activated seed culture liquid into a 1L Erlenmeyer flask with an inoculum size of 4%, the liquid volume of the fermentation medium is 300mL, and the formula of the fermentation medium is glucose 1g, yeast extract 1.5g, K 2 HPO 4 0.5g, water 100ml, pH 7.2. The fermentation conditions are temperature 30°C, rotation speed 160r / min, and cultivation time 36-38h.

[0043] 1.3 Preparation of bacteria...

Embodiment 3

[0053] Embodiment three embed photosynthetic bacteria

[0054] 1. Preparation of photosynthetic bacteria suspension

[0055] 1.1 Activation of photosynthetic bacteria

[0056] Pick a small amount of photosynthetic bacteria strains preserved on the slope, and insert them into a 100mL Erlenmeyer flask, and the seed medium filling volume is 20mL. The formula of the seed medium is 2 g of yeast extract, 0.5 g of potassium dihydrogen phosphate, 1 g of ammonium chloride, magnesium sulfate (MgSO 4 .7H 2 O) 0.5g, sodium acetate 2g, water 1000mL, pH 7.0. The activation conditions are a temperature of 30° C., a rotation speed of 160 r / min, and a culture time of 24 hours.

[0057] 1.2 Strain fermentation

[0058] Insert the activated seed culture solution into a 1L Erlenmeyer flask with an inoculum size of 4%, the liquid loading capacity of the fermentation medium is 300mL, and the fermentation medium formula is sodium acetate 1.5g, peptone 0.5g, sodium bicarbonate 0.6g, Yeast extra...

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Abstract

The invention discloses a transformation method for a bottom material of a sea cucumber breeding cofferdam. The transformation method comprises the following steps: S1, preparing oyster shell fragments of 1 to 2 cm, and performing sterilization processing; S2, embedding a bottom material transformation bacterium agent, namely, adding the oyster shell fragments and a bacterium suspension into a solution of sodium alginate and mixing uniformly, transferring the oyster shell fragments into the solution of CaCl2, and washing the solution of the CaCl2 which remains on oyster shells by using water through a cross-linking effect; S3, dispersing and settling the oyster shell fragments of the step S2 to the bottom of the sea cucumber breeding cofferdam. According to the transformation method, the bottom material environment of the sea cucumber breeding cofferdam can be effectively improved; the transformation method has the characteristics of environment protection, energy conservation, low cost and easiness in operation.

Description

technical field [0001] The invention relates to the transformation of the sea cucumber culture environment, more specifically, to the transformation of the sea cucumber culture cofferdam bottom quality. Background technique [0002] With the expansion of the scale of sea cucumber cultivation in my country and the improvement of the cultivation density, after several cycles of cultivation in the sea cucumber cultivation cofferdam, due to the large amount of surplus bait settling to the bottom of the cultivation cofferdam, sea cucumber excrement and algae, animals and plants in the cultivation cofferdam After death, it settles to the bottom, so the organic matter accumulated at the bottom of the breeding cofferdam increases with time. The decomposition of organic matter settled at the bottom of the cofferdam requires a large amount of oxygen, which leads to the lack of oxygen in the sea cucumber cofferdam, and some harmful intermediates are produced during the incomplete decomp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/30
CPCY02A40/81
Inventor 包卫洋侯英雪车全杨美圆王祖哲
Owner DALIAN XINYULONG OCEAN TREASURES
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