Biosensing method for detecting leukemia by graphene/mimetic peroxidase double-signal amplification

A technology of graphene and leukemia cells, which is applied in the medical basic application field of biosensors, to achieve the effects of improving sensitivity and specificity, sensitive detection, and short detection time

Inactive Publication Date: 2015-05-06
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, HGNs can be used as a nanomaterial with peroxidase catalytic activity in the preparation of biosensors, but the application of HGNs in the detection of tumor cells has not been reported yet.

Method used

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  • Biosensing method for detecting leukemia by graphene/mimetic peroxidase double-signal amplification
  • Biosensing method for detecting leukemia by graphene/mimetic peroxidase double-signal amplification
  • Biosensing method for detecting leukemia by graphene/mimetic peroxidase double-signal amplification

Examples

Experimental program
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Effect test

Embodiment 1

[0054] Example 1. Preparation of a kit for leukemia cell detection

[0055] The kit for the detection of leukemia cells contains magnetic bead capture probes and aptamer functionalized Hemin-graphene composite nanomaterials.

[0056] 1. Preparation of magnetic bead capture probes

[0057] (1) Preparation of biotin-labeled capture probes

[0058] The biotin-labeled capture probe is a biotin-labeled single-stranded DNA molecule, which is a product of Shanghai Shenggong Company, China. The single-stranded DNA sequence used by the probe is as follows:

[0059] 5'-ATCTAACTGCTGCGCCGCCGGGAAAATACTGTACGGTTAGAAAAAA-3' (SEQ ID NO: 1), wherein the biotin-labeled molecule is at the 3' end of the nucleic acid chain.

[0060] (2) Magnetic beads labeled with streptavidin

[0061] The streptavidin-labeled magnetic beads are Dynabeads streptavidin magnetic beads M280 produced by Invitrogen Company, and the specific parameters of the magnetic beads are as follows: such as magnetic beads with ...

Embodiment 2

[0098] Embodiment 2, the application example of the kit for leukemia cell detection

[0099] 1. Use the kit of Example 1 to detect whether the cells to be tested are leukemia cells

[0100] Cell samples to be tested: CEM cells (human acute T lymphocytic leukemia cells), Ramos cells (human B lymphocytic tumor cells).

[0101] Referring to the method described in Step 3A of Example 1, the cell samples to be tested were detected, and Ramos cells (human B lymphoma cells) were set as negative control groups and blank control groups without adding any cells in the experiment.

[0102] The results showed that the CEM cell group had an obvious color reaction (that is, the reaction system changed from colorless to blue), while the other groups, like the blank control group, had no color reaction. The result shows that the kit provided by the invention can realize the specific detection of leukemia cells.

[0103] Two, adopt the kit of embodiment 1 to detect the content of leukemia ce...

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Abstract

The invention discloses a biosensing method for detecting leukemia by graphene / mimetic peroxidase double-signal amplification. The invention provides a kit for detecting leukemia cells; the kit comprises (a) a magnetic bead capture probe and (b) an aptamer functionalization Hemin-graphene composite nanomaterial, wherein the magnetic bead capture probe comprises a magnetic bead and a capture probe fixed on the magnetic bead; the capture probe is a single-stranded deoxyribonucleic acid (DNA) molecule shown by sequence 1; the aptamer functionalization Hemin-graphene composite nanomaterial is composed of graphene modified by Hemin, and an aptamer probe fixed on the graphene modified by the Hemin; the aptamer probe is a single-stranded DNA molecule shown by sequence 2. After the biosensing method is used, the target leukemia cells with lower concentration can be captured and enriched within a short time, and a detection signal is amplified, so that the leukemia cells can be rapidly, accurately and sensitively detected.

Description

technical field [0001] The invention belongs to the medical basic application field of biosensors, and relates to a biosensing method for amplifying and detecting leukemia by graphene / peroxidase-like dual signals. Background technique [0002] Leukemia is a common and frequent hematopoietic stem cell clonal malignant disease. It is one of the top ten high-incidence malignant tumors in my country. It ranks first in the mortality rate of malignant tumors in people under the age of 35, and its incidence is increasing year by year. Its diagnostic classification was originally the FAB classification proposed by the French (F), American (A), and British (B) collaborative groups in 1976. It is a traditional method for diagnosing leukemia. With the application of cytogenetics and immune typing in the diagnosis of leukemia, the cytomorphological (Morphoiogic), immunological (Immunologic), and cytogenetic (Cytogentic) MIC typing of leukemia was proposed. Since the 1980s, due to the c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/553
CPCG01N33/553G01N33/57426
Inventor 赵永祥苏婧卢小玲黄勇
Owner GUANGXI MEDICAL UNIVERSITY
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